Mutation of OsSAC3, Encoding the Xanthine Dehydrogenase, Caused Early Senescence in Rice
Abstract
:1. Introduction
2. Results
2.1. Map-Based Clone and Identification of OsSAC3
2.2. Bioinformatic Analysis of OsSAC3
2.3. OsSAC3 Encodes a Cytoplasmic Xanthine Dehydrogenase with Constitutively Expression Pattern
2.4. Transcriptome and Gene Ontology Enrichment Analysis
2.5. Gene Expression Related to Purine Metabolism Was Altered in ossac3
2.6. Redox Process Was Altered in ossac3
2.7. Photosynthesis Was Damaged in ossac3
2.8. Mutation of OsSAC3 Alters Expression of Sugar Metabolism/Distribution Related Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Fine Mapping and Isolation of the OsSAC3 Gene
4.3. Physical and Chemical Analysis
4.4. Multiple Sequence Alignment and Evolutionary Analysis
4.5. Vector Construction, Transformation and Identification
4.6. Gene Expression Analysis
4.7. Transcriptome and KEGG Pathway Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xie, Z.; Zhao, B.; Zhang, M.; Sang, X.; Zhao, F.; Feng, P.; He, G.; Zhu, X. Mutation of OsSAC3, Encoding the Xanthine Dehydrogenase, Caused Early Senescence in Rice. Int. J. Mol. Sci. 2022, 23, 11053. https://doi.org/10.3390/ijms231911053
Xie Z, Zhao B, Zhang M, Sang X, Zhao F, Feng P, He G, Zhu X. Mutation of OsSAC3, Encoding the Xanthine Dehydrogenase, Caused Early Senescence in Rice. International Journal of Molecular Sciences. 2022; 23(19):11053. https://doi.org/10.3390/ijms231911053
Chicago/Turabian StyleXie, Ziyu, Bingbing Zhao, Mengxue Zhang, Xianchun Sang, Fangming Zhao, Ping Feng, Guanghua He, and Xiaoyan Zhu. 2022. "Mutation of OsSAC3, Encoding the Xanthine Dehydrogenase, Caused Early Senescence in Rice" International Journal of Molecular Sciences 23, no. 19: 11053. https://doi.org/10.3390/ijms231911053