Transcriptome Analysis Reveals the Complex Regulatory Pathway of Background Color in Juvenile Plectropomus leopardus Skin Color Variation
Abstract
:1. Introduction
2. Results
2.1. The Types of Chromatophores in P. leopardus under Different Backgrounds
2.2. Overview of RNA Sequencing Data
2.3. Characterization of the Skin mRNAs
2.4. DEG Analysis among the Four Groups
2.5. GO and KEGG Enrichment Analyses of the DEGs
2.6. qRT-PCR Verification
3. Discussion
4. Materials and Methods
4.1. Experiment, Fish Sampling, and Tissue Preparation
4.2. Total RNA Extraction and Qualification
4.3. cDNA Library Construction and Sequencing
4.4. Quality Control, Mapping, and Assembly
4.5. Differently Expressed Genes (DEGs) Analysis
4.6. Functional Annotation and Pathway Enrichment
4.7. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Validation
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Song, F.; Wang, L.; Yang, Z.; Shi, L.; Zheng, D.; Zhang, K.; Sun, J.; Luo, J. Transcriptome Analysis Reveals the Complex Regulatory Pathway of Background Color in Juvenile Plectropomus leopardus Skin Color Variation. Int. J. Mol. Sci. 2022, 23, 11186. https://doi.org/10.3390/ijms231911186
Song F, Wang L, Yang Z, Shi L, Zheng D, Zhang K, Sun J, Luo J. Transcriptome Analysis Reveals the Complex Regulatory Pathway of Background Color in Juvenile Plectropomus leopardus Skin Color Variation. International Journal of Molecular Sciences. 2022; 23(19):11186. https://doi.org/10.3390/ijms231911186
Chicago/Turabian StyleSong, Feibiao, Lei Wang, Zihang Yang, Liping Shi, Da Zheng, Kaixi Zhang, Junlong Sun, and Jian Luo. 2022. "Transcriptome Analysis Reveals the Complex Regulatory Pathway of Background Color in Juvenile Plectropomus leopardus Skin Color Variation" International Journal of Molecular Sciences 23, no. 19: 11186. https://doi.org/10.3390/ijms231911186