Analysis of Long Non-Coding RNA (lncRNA) UCA1, MALAT1, TC0101441, and H19 Expression in Endometriosis
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. RNA Isolation
4.3. Spectrophotometric Analysis of Purity and Concentration of RNA
4.4. RT-PCR Reaction
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patients | Control | |
---|---|---|
N | 100 | 100 |
Mean age (range) years | 36.4 (23–58) | 38.3 (29–61) |
Body mass index (BMI) (kg/m2) | ||
<25 | 44 (44%) | 42 (42%) |
25 ≤ BMI < 30 | 48 (48%) | 49 (49%) |
≥30 | 8 (8%) | 9 (9%) |
Parity > 0 | ||
yes | 46 (46%) | 49 (49%) |
1 | 29 (29%) | 31 (31%) |
2 and more | 17 (17%) | 18 (18%) |
no | 54 (54%) | 61 (61%) |
Spontaneous abortions | ||
yes (spontaneous) | 10 (10%) | 2 (2%) |
no | 90 (90%) | 98 (98%) |
Use of hormone replacement therapy (HRT) | ||
yes | 21 (21%) | 14 (14%) |
no | 79 (79%) | 86 (86%) |
Clinical stage (rASRM) * | ||
I | 11 (11%) | |
II | 14 (14%) | |
III | 44 (44%) | |
IV | 31 (31%) |
Mann–Whitney U Test | |||
---|---|---|---|
lncRNA | p | N-Patients | N-Controls |
UCA1 | 0.845 | 100 | 100 |
MALAT1 | 0.170 | 100 | 100 |
TC0101441 | 0.850 | 100 | 100 |
H19 | 0.003 | 100 | 100 |
Spearman’s Correlation | |||
---|---|---|---|
lncRNA | R-Spearman | The Two-Sample t-Test T (N-2) | p |
UCA1 & staging | 0.131 | 1.10 | 0.062 |
MALAT1 & staging | 0.100 | 1.68 | 0.068 |
TC0101441 & staging | 0.120 | 0.84 | 0.204 |
H19 & staging | 0.240 | 2.625 | 0.022 |
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Szaflik, T.; Romanowicz, H.; Szyłło, K.; Kołaciński, R.; Michalska, M.M.; Samulak, D.; Smolarz, B. Analysis of Long Non-Coding RNA (lncRNA) UCA1, MALAT1, TC0101441, and H19 Expression in Endometriosis. Int. J. Mol. Sci. 2022, 23, 11583. https://doi.org/10.3390/ijms231911583
Szaflik T, Romanowicz H, Szyłło K, Kołaciński R, Michalska MM, Samulak D, Smolarz B. Analysis of Long Non-Coding RNA (lncRNA) UCA1, MALAT1, TC0101441, and H19 Expression in Endometriosis. International Journal of Molecular Sciences. 2022; 23(19):11583. https://doi.org/10.3390/ijms231911583
Chicago/Turabian StyleSzaflik, Tomasz, Hanna Romanowicz, Krzysztof Szyłło, Radosław Kołaciński, Magdalena M. Michalska, Dariusz Samulak, and Beata Smolarz. 2022. "Analysis of Long Non-Coding RNA (lncRNA) UCA1, MALAT1, TC0101441, and H19 Expression in Endometriosis" International Journal of Molecular Sciences 23, no. 19: 11583. https://doi.org/10.3390/ijms231911583