Effect of DSS-Induced Ulcerative Colitis and Butyrate on the Cytochrome P450 2A5: Contribution of the Microbiome
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Animals and Experimental Design
4.2. Preparation of Microsomal Fraction
4.3. Cytochrome P450 2A Enzyme Activity Assays
4.4. RNA Isolation and Quantitative Real-Time RT-PCR (qRT-PCR)
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Satka, S.; Frybortova, V.; Zapletalova, I.; Anzenbacher, P.; Anzenbacherova, E.; Kozakova, H.; Srutkova, D.; Hudcovic, T.; Jourova, L. Effect of DSS-Induced Ulcerative Colitis and Butyrate on the Cytochrome P450 2A5: Contribution of the Microbiome. Int. J. Mol. Sci. 2022, 23, 11627. https://doi.org/10.3390/ijms231911627
Satka S, Frybortova V, Zapletalova I, Anzenbacher P, Anzenbacherova E, Kozakova H, Srutkova D, Hudcovic T, Jourova L. Effect of DSS-Induced Ulcerative Colitis and Butyrate on the Cytochrome P450 2A5: Contribution of the Microbiome. International Journal of Molecular Sciences. 2022; 23(19):11627. https://doi.org/10.3390/ijms231911627
Chicago/Turabian StyleSatka, Stefan, Veronika Frybortova, Iveta Zapletalova, Pavel Anzenbacher, Eva Anzenbacherova, Hana Kozakova, Dagmar Srutkova, Tomas Hudcovic, and Lenka Jourova. 2022. "Effect of DSS-Induced Ulcerative Colitis and Butyrate on the Cytochrome P450 2A5: Contribution of the Microbiome" International Journal of Molecular Sciences 23, no. 19: 11627. https://doi.org/10.3390/ijms231911627