Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14
Abstract
:1. Introduction
2. Results
2.1. Characterization of PgrABCG Genes and the Encoded Proteins
2.2. Phylogeny and Structure Analysis of PgrABCG Genes
2.3. Expression Patterns of PgrABCG Genes in Pomegranate Tissues
2.4. Co-Expression, and Evolution Analysis of the Candidate Genes
2.5. Functional Analysis of PgrABCG14
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Sequence Analysis of PgrABCG Genes and the Encoded Proteins
4.3. Conserved Motifs and Gene Structure Analysis
4.4. Phylogenetic Analysis of ABCG Proteins in Four Species
4.5. Expression Analysis of PgrABCG Genes in Different Tissues
4.6. Semi-Quantitative PCR and Quantitative Real Time Polymerase Chain Reaction (qRT-PCR) Analysis
4.7. Co-Expression Analysis
4.8. Evolutionary Analysis of Candidate PgrABCG Genes
4.9. Vector Construction and Plant Transformation
4.10. GUS Histochemical Analysis
4.11. Exogenous Cytokinin Treatment
4.12. Lignin Histochemical Staining and Content Determination
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yu, Q.; Li, J.; Qin, G.; Liu, C.; Cao, Z.; Jia, B.; Xu, Y.; Li, G.; Yang, Y.; Su, Y.; et al. Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14. Int. J. Mol. Sci. 2022, 23, 11661. https://doi.org/10.3390/ijms231911661
Yu Q, Li J, Qin G, Liu C, Cao Z, Jia B, Xu Y, Li G, Yang Y, Su Y, et al. Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14. International Journal of Molecular Sciences. 2022; 23(19):11661. https://doi.org/10.3390/ijms231911661
Chicago/Turabian StyleYu, Qing, Jiyu Li, Gaihua Qin, Chunyan Liu, Zhen Cao, Botao Jia, Yiliu Xu, Guixiang Li, Yuan Yang, Ying Su, and et al. 2022. "Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14" International Journal of Molecular Sciences 23, no. 19: 11661. https://doi.org/10.3390/ijms231911661