GDF11 Regulates PC12 Neural Stem Cells via ALK5-Dependent PI3K-Akt Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. GDF11 Mitigated Cell Viability and Proliferation
2.2. GDF11 Promoted Differentiation of PC12 Cells
2.3. GDF11 Stimulated Apoptosis of PC12 Cells
2.4. GDF11 Arrested Cell Cycle of PC12 Cells
2.5. GDF11 Attenuated PC12 Cell Migration
2.6. TMT–Based Protein Identification and Quantification
2.7. Annotation Analysis of the Differentially Expressed Proteins
2.8. PI3K-Akt Pathway Was Enriched by CSP100 PLUS Microarray
2.9. GDF11 Activated PI3K-Akt Signal Pathway
2.10. GDF11 Affected Neural Stem Cells through ALK5-Dependent PI3K-Akt Pathway
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Viability/Proliferation Assay
4.3. Cell Apoptosis Assay
4.4. Wound Healing/Migration Assay
4.5. qRT-PCR Analysis
4.6. Western Blot Analysis and Validation
4.7. Sample Preparation, TMT-Labeling, and LC-MS/MS
4.8. MS/MS Data Analysis and Bioinformatics Analysis
4.9. Profiles of Key Signaling Pathway by an Antibody Array
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Total Spectrums | Matched Spectrums | Peptides | Unique Peptides | Identified Proteins | Quantifiable Proteins | DEPs |
---|---|---|---|---|---|---|
784,796 | 79,915 | 18,504 | 17,878 | 5452 | 5209 | 165 |
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Wang, Z.; Jiang, P.; Liu, F.; Du, X.; Ma, L.; Ye, S.; Cao, H.; Sun, P.; Su, N.; Lin, F.; et al. GDF11 Regulates PC12 Neural Stem Cells via ALK5-Dependent PI3K-Akt Signaling Pathway. Int. J. Mol. Sci. 2022, 23, 12279. https://doi.org/10.3390/ijms232012279
Wang Z, Jiang P, Liu F, Du X, Ma L, Ye S, Cao H, Sun P, Su N, Lin F, et al. GDF11 Regulates PC12 Neural Stem Cells via ALK5-Dependent PI3K-Akt Signaling Pathway. International Journal of Molecular Sciences. 2022; 23(20):12279. https://doi.org/10.3390/ijms232012279
Chicago/Turabian StyleWang, Zongkui, Peng Jiang, Fengjuan Liu, Xi Du, Li Ma, Shengliang Ye, Haijun Cao, Pan Sun, Na Su, Fangzhao Lin, and et al. 2022. "GDF11 Regulates PC12 Neural Stem Cells via ALK5-Dependent PI3K-Akt Signaling Pathway" International Journal of Molecular Sciences 23, no. 20: 12279. https://doi.org/10.3390/ijms232012279