AMPK Amplifies IL2–STAT5 Signaling to Maintain Stability of Regulatory T Cells in Aged Mice
Abstract
:1. Introduction
2. Results
2.1. Young AMPKα1fl/flFoxp3yfp-cre (AMPK-KO) and Foxp3ypf-cre (WT) Mice Are Phenotypically Similar
2.2. Young AMPK-KO Mice Showed a Less Inflammatory Phenotype Than WT Mice
2.3. AMPK Loss Abolishes the Ability of Tregs to Resolve EAE in Aged Mice
2.4. Treg-Specific AMPK Deletion Develops Spontaneous Systemic Lymphoproliferative Disease with Age
2.5. Substantial Decrease of CD25 Expression in AMPK-KO Tregs with Age
2.6. AMPK Supports Suppressive Activity of Tregs
2.7. AMPK Amplifies IL-2–STAT5 Signaling in Tregs
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. EAE Models
4.3. Cell Purification and Culture
4.4. Flow Cytometry and Cell Sorting
4.5. Total RNA Isolation and Quantitative Real-Time PCR
4.6. T Cell Transfer Colitis
4.7. Histopathology
4.8. Enzyme-Linked Immunosorbent Assay (ELISA)
4.9. RNA Sequencing
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pokhrel, R.H.; Kang, B.; Timilshina, M.; Chang, J.-H. AMPK Amplifies IL2–STAT5 Signaling to Maintain Stability of Regulatory T Cells in Aged Mice. Int. J. Mol. Sci. 2022, 23, 12384. https://doi.org/10.3390/ijms232012384
Pokhrel RH, Kang B, Timilshina M, Chang J-H. AMPK Amplifies IL2–STAT5 Signaling to Maintain Stability of Regulatory T Cells in Aged Mice. International Journal of Molecular Sciences. 2022; 23(20):12384. https://doi.org/10.3390/ijms232012384
Chicago/Turabian StylePokhrel, Ram Hari, Ben Kang, Maheshwor Timilshina, and Jae-Hoon Chang. 2022. "AMPK Amplifies IL2–STAT5 Signaling to Maintain Stability of Regulatory T Cells in Aged Mice" International Journal of Molecular Sciences 23, no. 20: 12384. https://doi.org/10.3390/ijms232012384