Tissue Sheet Engineered Using Human Umbilical Cord-Derived Mesenchymal Stem Cells Improves Diabetic Wound Healing
Abstract
:1. Introduction
2. Results
2.1. Characterization of hUC-MSCs
2.2. hUC-MSC Tissue Sheet Construction and Evaluation
2.3. hUC-MSC Tissue Sheet Accelerates Diabetic Wound Healing
2.4. hUC-MSC Tissue Sheet Induces Collagen Synthesis In Vivo
2.5. hUC-MSC Tissue Sheet Remarkably Promotes New Blood Vessel Formation and Maturation in Diabetic Wounds
2.6. hUC-MSC Tissue Sheet Regulates the Inflammatory Response in Diabetic Wounds
3. Discussion
4. Materials and Methods
4.1. Construction of PLGA Scaffold
4.2. Culture of hUC-MSCs
4.3. Characterization of hUC-MSCs
4.4. hUC-MSC Tissue Sheet Formation
4.5. In Vivo Wound Healing Experiments in a db/db Mouse Model
4.6. Immunofluorescence and Histological Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
hUC-MSCs | human umbilical cord mesenchymal stem cells |
PLGA | poly(lactide-co-glycolic acid) |
ECM | extracellular matrix |
SEM | scanning electron microscope |
H&E | hematoxylin and eosin |
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Antibody Name | Dilution | Company | Catalog # |
---|---|---|---|
hCD31 | 1:1000 | Biolegend | 303106 |
hCD34 | 1:1000 | Biolegend | 343506 |
hCD45 | 1:1000 | Biolegend | 304008 |
hCD73 | 1:1000 | Biolegend | 344004 |
hCD90 | 1:1000 | Biolegend | 328110 |
hCD105 | 1:1000 | Biolegend | 323206 |
HLA-ABC | 1:1000 | Biolegend | 311406 |
HLA-DR | 1:1000 | Biolegend | 307606 |
HLA-G | 1:1000 | Biolegend | 335905 |
Vimentin [D21H3] | 1:100 | Cell Signaling | 5741S |
Collagen Type Ⅰ | 1:100 | Sigma-Aldrich | C2456 |
Collagen Type Ⅲ | 1:100 | Abcam | ab7778 |
CD31 | 1:100 | Abcam | ab28364 |
Actin (Smooth Muscle) | 1:100 | Dako | M0851 |
CD68 | 1:100 | Abcam | ab31630 |
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Zhang, J.; Qu, X.; Li, J.; Harada, A.; Hua, Y.; Yoshida, N.; Ishida, M.; Sawa, Y.; Liu, L.; Miyagawa, S. Tissue Sheet Engineered Using Human Umbilical Cord-Derived Mesenchymal Stem Cells Improves Diabetic Wound Healing. Int. J. Mol. Sci. 2022, 23, 12697. https://doi.org/10.3390/ijms232012697
Zhang J, Qu X, Li J, Harada A, Hua Y, Yoshida N, Ishida M, Sawa Y, Liu L, Miyagawa S. Tissue Sheet Engineered Using Human Umbilical Cord-Derived Mesenchymal Stem Cells Improves Diabetic Wound Healing. International Journal of Molecular Sciences. 2022; 23(20):12697. https://doi.org/10.3390/ijms232012697
Chicago/Turabian StyleZhang, Jingbo, Xiang Qu, Junjun Li, Akima Harada, Ying Hua, Noriko Yoshida, Masako Ishida, Yoshiki Sawa, Li Liu, and Shigeru Miyagawa. 2022. "Tissue Sheet Engineered Using Human Umbilical Cord-Derived Mesenchymal Stem Cells Improves Diabetic Wound Healing" International Journal of Molecular Sciences 23, no. 20: 12697. https://doi.org/10.3390/ijms232012697