Quetiapine Shortens the Lifespan of Caenorhabditis elegans through DOP-2, DAF-2 and RSKS-1
Abstract
:1. Introduction
2. Results
2.1. Quetiapine Reduces Healthspan but Does Not Affect the Locomotion and Pharyngeal Pumping of C. elegans
2.2. Quetiapine-Mediated Lifespan Shortening Is Dependent on DOP-2
2.3. Quetiapine Shortens Lifespan through DAF-2, but Not the Downstream Akt Pathway
2.4. Quetiapine-Induced Lifespan Shortening Is Independent of the AAK-2–DAF-16 Pathway
2.5. Quetiapine-Induced Lifespan Reduction Is Dependent on mTOR Signaling
2.6. Quetiapine-Induced Lifespan Reduction Is Associated with Mitochondrial Function
3. Discussion
4. Materials and Methods
4.1. Nematode C. Elegans Strains and Their Maintenance
4.2. Preparation of Reagents
4.3. Lifespan Assays
4.4. Phenotype Assays
4.5. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Genotype |
---|---|
N2 | Bristol, wild type |
CF1038 | daf-16(mu86) I |
CB1370 | daf-2(e1370) III |
CB4876 | clk-1(e2519) III |
LX702 | dop-2(vs105) V |
MQ887 | isp-1(qm150) IV |
RB1206 | rsks-1(ok1255) III |
RB759 | akt-1(ok525) V |
TG38 | aak-2(gt33) X |
TJ1052 | age-1(hx546) II |
VC204 | akt-2(ok393) X |
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Jiang, Y.; Gaur, U.; Cao, Z.; Hou, S.-T.; Zheng, W. Quetiapine Shortens the Lifespan of Caenorhabditis elegans through DOP-2, DAF-2 and RSKS-1. Int. J. Mol. Sci. 2022, 23, 12927. https://doi.org/10.3390/ijms232112927
Jiang Y, Gaur U, Cao Z, Hou S-T, Zheng W. Quetiapine Shortens the Lifespan of Caenorhabditis elegans through DOP-2, DAF-2 and RSKS-1. International Journal of Molecular Sciences. 2022; 23(21):12927. https://doi.org/10.3390/ijms232112927
Chicago/Turabian StyleJiang, Yizhou, Uma Gaur, Zhibai Cao, Sheng-Tao Hou, and Wenhua Zheng. 2022. "Quetiapine Shortens the Lifespan of Caenorhabditis elegans through DOP-2, DAF-2 and RSKS-1" International Journal of Molecular Sciences 23, no. 21: 12927. https://doi.org/10.3390/ijms232112927