Enriched Environment Attenuates Enhanced Trait Anxiety in Association with Normalization of Aberrant Neuro-Inflammatory Events
Abstract
:1. Introduction
2. Results
2.1. EE Housing Attenuates Anxiety Behavior in HAB
2.2. EE-Induced Anxiolysis Is Associated with Attenuation of Aberrant Microglia in HABs within the Anterior and Posterior DG
2.3. EE-Induced Anxiolysis Is Associated with Attenuation of Phagocytic Microglial Density in HABs within the DG
2.4. EE-Induced Anxiolysis Is Associated with Attenuation of Upregulated Microglia in HABs within the mPFC
3. Discussion
3.1. EE-Mediated Anxiolysis in HAB Mice
3.2. EE Attenuates Aberrant Microglial Phenotype in the HAB Mice
3.3. Effects of EE on Microglial CD68-Mediated Phagocytosis in HAB Mice
4. Materials and Methods
4.1. Animals
4.2. Enriched Environment
4.3. Open-Field Test
4.4. Light–Dark Test
4.5. Immunohistochemistry
4.6. Immunofluorescence Microscopy
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sah, A.; Rooney, S.; Kharitonova, M.; Sartori, S.B.; Wolf, S.A.; Singewald, N. Enriched Environment Attenuates Enhanced Trait Anxiety in Association with Normalization of Aberrant Neuro-Inflammatory Events. Int. J. Mol. Sci. 2022, 23, 13052. https://doi.org/10.3390/ijms232113052
Sah A, Rooney S, Kharitonova M, Sartori SB, Wolf SA, Singewald N. Enriched Environment Attenuates Enhanced Trait Anxiety in Association with Normalization of Aberrant Neuro-Inflammatory Events. International Journal of Molecular Sciences. 2022; 23(21):13052. https://doi.org/10.3390/ijms232113052
Chicago/Turabian StyleSah, Anupam, Sinead Rooney, Maria Kharitonova, Simone B. Sartori, Susanne A. Wolf, and Nicolas Singewald. 2022. "Enriched Environment Attenuates Enhanced Trait Anxiety in Association with Normalization of Aberrant Neuro-Inflammatory Events" International Journal of Molecular Sciences 23, no. 21: 13052. https://doi.org/10.3390/ijms232113052