Antifibrotic TSG-6 Expression Is Synergistically Increased in Both Cells during Coculture of Mesenchymal Stem Cells and Macrophages via the JAK/STAT Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Increased Expression of TSG-6 mRNA in ASCs after Coculture with Macrophages
2.2. TSG-6 Expression in ASCs and Macrophages Treated with Cytokine Combinations
2.3. Activation of the Signal Transducer and Activator of Transcription (STAT) Signaling Pathway for TSG-6 Expression
2.4. Antifibrotic Effects of TSG-6 Expressed in ASCs and Macrophages
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Macrophage Differentiation of THP-1 Monocytes
4.4. qPCR
4.5. Immunoblotting
4.6. Small Interfering RNA (siRNA) Treatment
4.7. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gong, S.C.; Yoon, Y.; Jung, P.Y.; Kim, M.Y.; Baik, S.K.; Ryu, H.; Eom, Y.W. Antifibrotic TSG-6 Expression Is Synergistically Increased in Both Cells during Coculture of Mesenchymal Stem Cells and Macrophages via the JAK/STAT Signaling Pathway. Int. J. Mol. Sci. 2022, 23, 13122. https://doi.org/10.3390/ijms232113122
Gong SC, Yoon Y, Jung PY, Kim MY, Baik SK, Ryu H, Eom YW. Antifibrotic TSG-6 Expression Is Synergistically Increased in Both Cells during Coculture of Mesenchymal Stem Cells and Macrophages via the JAK/STAT Signaling Pathway. International Journal of Molecular Sciences. 2022; 23(21):13122. https://doi.org/10.3390/ijms232113122
Chicago/Turabian StyleGong, Seong Chan, Yongdae Yoon, Pil Young Jung, Moon Young Kim, Soon Koo Baik, Hoon Ryu, and Young Woo Eom. 2022. "Antifibrotic TSG-6 Expression Is Synergistically Increased in Both Cells during Coculture of Mesenchymal Stem Cells and Macrophages via the JAK/STAT Signaling Pathway" International Journal of Molecular Sciences 23, no. 21: 13122. https://doi.org/10.3390/ijms232113122