Identification of Low-Nitrogen-Related miRNAs and Their Target Genes in Sugarcane and the Role of miR156 in Nitrogen Assimilation
, Yachun Su 1, Jinlong Guo 1, Youxiong Que 1 and Liping Xu 1,*Round 1
Reviewer 1 Report
This manuscript describes the miRNA analysis of a sugarcane variety ROC22 considered to be N-efficient. Here, the authors made a good effort to understand the miRNA responses of ROC22 to low N supply and identified miRNAs associated with low N tolerance in this variety. Then they generated sugarcane miRNA transgenic lines of Arabidopsis and demonstrated the proposed the low N tolerance function of miRNA. The experiments are well-conducted, and the conclusions are backed by the data presented. I recommend that it be considered for publication.
Author Response
Dear Reviewer,
Thank you very much for reviewing our manuscript carefully. Thank you very much for your affirmation and approval to our article.
Reviewer 2 Report
The manuscript presents original research that fits within the scope of the journal.
There is no information on proposed future studies at the end of the conclusions.
Please check the linguistic expression carefully in all parts of the article.
The manuscript contains grammatical mistakes/errors and should be improved thoroughly.
Author Response
Dear Reviewer,
Thank you very much for reviewing our manuscript promptly. We are very grateful to you for the helpful suggestions. We have taken into account the kind suggestions and revised the manuscript according to the suggestions. The main revisions were marked up by“Track Changes” in the revised MS. Thanks again for your advice. We hope to learn more from you. The questions raised by you are answered and explained as follows:
Q1: There is no information on proposed future studies at the end of the conclusions.
Answer: Thanks for the kind suggestion! We have added future research plans in the conclusion section.
Q2: Please check the linguistic expression carefully in all parts of the article.
Answer: Thank you very much for the kind suggestion! We have modified it accordingly.
Q3: The manuscript contains grammatical mistakes/errors and should be improved thoroughly.
Answer: Thanks for the kind suggestion! We have modified it accordingly.
