The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla
Abstract
:1. Introduction
2. Results
2.1. Identification and Analysis of DEGs
2.2. Identification and Expression Patterns of Known miRNAs
2.3. qRT-PCR Validation of Related Genes and miRNA
2.4. Prediction and Enrichment Analysis of the Target Genes of Differentially Expressed miRNAs
2.5. Gene Cloning and Gene Structure Analysis
2.6. Functional Analysis of PnTCP2
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Construction of Small RNA Libraries and Deep Sequencing
4.3. Analysis of Sequencing Data
4.4. Analysis of DEGs and Differentially Expressed miRNAs
4.5. Known miRNA Alignment, Target Prediction, and GO and KEGG Pathway Analyses
4.6. Quantitative Real-Time PCR Analysis
4.7. Protein Sequence Analysis
4.8. Vector Construction
4.9. Protein Interaction Verification
4.10. TRV-VIGS Gene Silencing
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sun, B.; He, X.; Long, F.; Yu, C.; Fei, Y. The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla. Int. J. Mol. Sci. 2022, 23, 13296. https://doi.org/10.3390/ijms232113296
Sun B, He X, Long F, Yu C, Fei Y. The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla. International Journal of Molecular Sciences. 2022; 23(21):13296. https://doi.org/10.3390/ijms232113296
Chicago/Turabian StyleSun, Bing, Xinru He, Fengying Long, Cui Yu, and Yongjun Fei. 2022. "The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla" International Journal of Molecular Sciences 23, no. 21: 13296. https://doi.org/10.3390/ijms232113296