The Inhibition of the Inducible Nitric Oxide Synthase Enhances the DPSC Mineralization under LPS-Induced Inflammation
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Synthesis of iNOS Inhibitors
3.2. Cell Culture
3.3. Establishment of Pro-Inflammatory Conditions and Cell Exposures to Inhibitors
3.4. Lactate Dehydrogenase (LDH) Release
3.5. Interleukin-6 Release
3.6. Immunophenotype In Vitro by Flow Cytometry
3.7. Extracellular Matrix Mineralization (Alizarin Red Staining)
3.8. ELISA Analysis of VEGF Secretion
3.9. Statistics
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cpd | iNOS IC50 (nM) | eNOS/iNOS Selectivity | logP * | TPSA * |
---|---|---|---|---|
1400W | 81 | 3800 | 1.06 | 61.90 |
CM544 | 56 | 4569 | 1.31 | 68.22 |
FAB1020 | 11 | >900 | 2.70 | 64.98 |
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Cataldi, A.; Amoroso, R.; di Giacomo, V.; Zara, S.; Maccallini, C.; Gallorini, M. The Inhibition of the Inducible Nitric Oxide Synthase Enhances the DPSC Mineralization under LPS-Induced Inflammation. Int. J. Mol. Sci. 2022, 23, 14560. https://doi.org/10.3390/ijms232314560
Cataldi A, Amoroso R, di Giacomo V, Zara S, Maccallini C, Gallorini M. The Inhibition of the Inducible Nitric Oxide Synthase Enhances the DPSC Mineralization under LPS-Induced Inflammation. International Journal of Molecular Sciences. 2022; 23(23):14560. https://doi.org/10.3390/ijms232314560
Chicago/Turabian StyleCataldi, Amelia, Rosa Amoroso, Viviana di Giacomo, Susi Zara, Cristina Maccallini, and Marialucia Gallorini. 2022. "The Inhibition of the Inducible Nitric Oxide Synthase Enhances the DPSC Mineralization under LPS-Induced Inflammation" International Journal of Molecular Sciences 23, no. 23: 14560. https://doi.org/10.3390/ijms232314560