Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas
Abstract
:1. Introduction
2. Results and Discussion
2.1. Biomass Accumulation of I. batatas Calli under Stress Factors and Chemical Elicitor Treatment
2.2. Accumulation of Phytochemicals in I. batatas Calli under Various Stimuli
2.3. Expression Pattern of Biosynthetic Genes in I. batatas Calli
2.4. Analysis of IbT-DNA2 Genes Expression in I. batatas Calli
2.5. Effect of Ib-rolB/C Gene Expression on Secondary Metabolism of Transgenic I. batatas Calli
3. Materials and Methods
3.1. Plant Cell Cultures and Treatments
3.2. High-Performance Liquid Chromatography (HPLC) Analysis
3.2.1. Chemicals
3.2.2. Sample Preparation for Analytical Chromatography
3.2.3. Analytical Chromatography and Mass-Spectrometry
3.3. Obtaining Ib-rolB/C–Expressing Cell Cultures
3.4. Synthesis of cDNA and Real-Time PCR Analysis
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Callus Line | CQAs Content, mg/g DW | Dry Weight (g/L) | CQAs Production, mg/L | ||||
---|---|---|---|---|---|---|---|
CGA | 3,5-diCQA | Total CQAs | CGA | 3,5-diCQA | Total CQAs | ||
Control | 0.56 ± 0.05 | 3.23 ± 0.08 | 4.55 ± 0.47 | 14.67 ± 0.29 | 8.23 ± 0.16 | 47.40 ± 0.95 | 66.78 ± 1.34 |
15 °C | 0.14 ± 0.02 | 0.58 ± 0.08 | 0.98 ± 0.08 | 0.88 ± 0.28 | 0.13 ± 0.04 | 0.51 ± 0.16 | 0.86 ± 0.27 |
30 °C | 1.10 ± 0.14 | 5.29 ± 0.74 | 7.34 ± 0.78 | 15.01 ± 0.62 | 16.58 ± 0.68 | 79.39 ± 3.27 | 110.14 ± 4.53 |
Normal light * | 0.29 ± 0.04 | 1.99 ± 0.28 | 2.68 ± 0.29 | 11.47 ± 0.28 | 3.35 ± 0.09 | 22.83 ± 0.58 | 30.74 ± 0.79 |
High light (7 d) ** | 0.22 ± 0.03 | 1.40 ± 0.20 | 1.97 ± 0.20 | 3.34 ± 0.14 | 0.75 ± 0.03 | 4.68 ± 0.21 | 6.59 ± 0.29 |
High light (14 d) *** | 0.25 ± 0.03 | 1.33 ± 0.19 | 1.90 ± 0.19 | 7.46 ± 0.15 | 1.87 ± 0.04 | 9.90 ± 0.19 | 14.16 ± 0.28 |
UV 10 min | 0.81 ± 0.10 | 5.50 ± 0.77 | 7.33 ± 0.81 | 14.67 ± 0.29 | 11.84 ± 0.24 | 80.76 ± 1.62 | 107.51 ± 2.15 |
UV 60 min | 0.52 ± 0.07 | 4.15 ± 0.58 | 5.41 ± 0.61 | 14.67 ± 0.29 | 7.64 ± 0.15 | 60.93 ± 1.22 | 79.42 ± 1.59 |
SA 10 μM | 1.00 ± 0.13 | 8.59 ± 1.20 | 11.59 ± 1.25 | 27.25 ± 0.39 | 27.24 ± 0.39 | 234.08 ± 3.39 | 315.83 ± 4.58 |
SA 50 μM | 0.85 ± 0.11 | 6.88 ± 0.96 | 9.35 ± 1.00 | 19.65 ± 1.10 | 16.74 ± 0.94 | 135.08 ± 7.54 | 183.76 ± 10.26 |
SA 100 μM | 0.48 ± 0.06 | 3.60 ± 0.50 | 4.77 ± 0.53 | 17.57 ± 0.82 | 8.39 ± 0.39 | 63.34 ± 2.97 | 83.91 ± 3.94 |
MeJA 10 μM | 0.85 ± 0.11 | 7.52 ± 1.05 | 9.85 ± 1.11 | 7.36 ± 1.23 | 6.24 ± 1.05 | 55.34 ± 9.28 | 72.51 ± 12.15 |
MeJA 50 μM | 1.22 ± 0.16 | 14.47 ± 2.03 | 18.32 ± 2.14 | 5.24 ± 0.75 | 6.41 ± 0.92 | 75.82 ± 10.85 | 96.01 ± 13.74 |
MeJA 100 μM | 0.91 ± 0.12 | 9.87 ± 1.38 | 13.66 ± 1.43 | 3.14 ± 0.78 | 2.86 ± 0.71 | 31.02 ± 7.72 | 42.95 ± 10.69 |
SNP 10 μM | 0.76 ± 0.10 | 5.66 ± 0.79 | 7.57 ± 0.83 | 17.84 ± 0.90 | 13.47 ± 0.68 | 101.03 ± 5.08 | 135.11 ± 6.79 |
SNP 50 μM | 0.38 ± 0.05 | 1.98 ± 0.28 | 2.69 ± 0.29 | 10.87 ± 0.30 | 4.10 ± 0.11 | 21.54 ± 0.59 | 29.28 ± 0.80 |
SNP 100 μM | 0.38 ± 0.05 | 1.98 ± 0.28 | 2.65 ± 0.29 | 3.39 ± 0.31 | 1.29 ± 0.12 | 6.71 ± 0.62 | 8.96 ± 0.82 |
↓ > 2 | ↓ < 2 | 1 | ↑ < 2 | ↑ > 2 |
Ib-rolB/C | ORF13 | ORF14, ORF17n, ORF18/17n | |
---|---|---|---|
Control | UD | UD | UD |
15 °C | UD | 1.07 ± 0.08 | UD |
30 °C | UD | UD | UD |
Normal light * | 5.17 ± 0.12 | UD | UD |
High light ** | UD | UD | UD |
UV 10 min | 2.49 ± 0.09 | UD | UD |
UV 60 min | 3.21 ± 0.11 | UD | UD |
Chemical elicitors (MeJA, SA, and SNP) | UD | UD | UD |
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Vasyutkina, E.A.; Yugay, Y.A.; Grigorchuk, V.P.; Grishchenko, O.V.; Sorokina, M.R.; Yaroshenko, Y.L.; Kudinova, O.D.; Stepochkina, V.D.; Bulgakov, V.P.; Shkryl, Y.N. Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas. Int. J. Mol. Sci. 2022, 23, 15100. https://doi.org/10.3390/ijms232315100
Vasyutkina EA, Yugay YA, Grigorchuk VP, Grishchenko OV, Sorokina MR, Yaroshenko YL, Kudinova OD, Stepochkina VD, Bulgakov VP, Shkryl YN. Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas. International Journal of Molecular Sciences. 2022; 23(23):15100. https://doi.org/10.3390/ijms232315100
Chicago/Turabian StyleVasyutkina, Elena A., Yulia A. Yugay, Valeria P. Grigorchuk, Olga V. Grishchenko, Maria R. Sorokina, Yulia L. Yaroshenko, Olesya D. Kudinova, Varvara D. Stepochkina, Victor P. Bulgakov, and Yury N. Shkryl. 2022. "Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas" International Journal of Molecular Sciences 23, no. 23: 15100. https://doi.org/10.3390/ijms232315100