Effects of Apamin on MPP+-Induced Calcium Overload and Neurotoxicity by Targeting CaMKII/ERK/p65/STAT3 Signaling Pathways in Dopaminergic Neuronal Cells
Abstract
:1. Introduction
2. Results
2.1. APM Protects SH-SY5Y Cells and Rat Embryo Primary Mesencephalic Neurons against MPP+-Induced Neurotoxicity
2.2. APM Ameliorates MPP+-Induced Mitochondrial-Dependent Apoptosis Pathway in SH-SY5Y and Rat Embryo Primary Mesencephalic Neurons
2.3. APM, a Strong Inhibiter of SK2 Channel, Protects MPP+-Induced Loss of TH-Positive Neurons and Accumulation of αSYN via Ca2+ Signaling
2.4. APM Alleviates MPP+-Induced Neuroinflammatory Response, Oxidative and ER Stress
2.5. APM Ameliorates MPP+-Induced Neurotoxicity via ERK/STAT/p65 Signaling Pathway in Dopaminergic Neuronal Cells
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and Reagents
4.2. Morphology Examination
4.3. Cytotoxicity Assay
4.4. Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis
4.5. Enzyme-Linked Immunosorbent Assay (ELISA)
4.6. Immunoblot Analysis
4.7. Terminal Deoxynucleotidyl-Transferase-Mediated dUTP Nick End Labelling (TUNEL) Staining
4.8. JC-1 Mitochondrial Transmembrane Potential Assay
4.9. Detection of Intracellular Ca2+ and ROS Expression
4.10. Immunofluorescent Staining
4.11. Electrophoretic Mobility Shift Analysis
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Park, J.; Jang, K.M.; Park, K.-K. Effects of Apamin on MPP+-Induced Calcium Overload and Neurotoxicity by Targeting CaMKII/ERK/p65/STAT3 Signaling Pathways in Dopaminergic Neuronal Cells. Int. J. Mol. Sci. 2022, 23, 15255. https://doi.org/10.3390/ijms232315255
Park J, Jang KM, Park K-K. Effects of Apamin on MPP+-Induced Calcium Overload and Neurotoxicity by Targeting CaMKII/ERK/p65/STAT3 Signaling Pathways in Dopaminergic Neuronal Cells. International Journal of Molecular Sciences. 2022; 23(23):15255. https://doi.org/10.3390/ijms232315255
Chicago/Turabian StylePark, Jihyun, Kyung Mi Jang, and Kwan-Kyu Park. 2022. "Effects of Apamin on MPP+-Induced Calcium Overload and Neurotoxicity by Targeting CaMKII/ERK/p65/STAT3 Signaling Pathways in Dopaminergic Neuronal Cells" International Journal of Molecular Sciences 23, no. 23: 15255. https://doi.org/10.3390/ijms232315255