Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses
Abstract
:1. Introduction
2. Results
2.1. Screening Differentially Expressed Genes and Enrichment Analysis in ALI
2.2. Exploration and Identification of Key Genes in ALI Regulated by E. bodinieri Vaniot
2.3. EBE Alleviated Oxidative Stress of Cells Model by NQO1
2.4. EBE Inhibited Pyroptosis of Cells Model by BCL2
2.5. EBE Attenuated Inflammation of Cells Model by PTGS2
3. Discussion
4. Materials and Methods
4.1. ALI Gene Expression Data Acquisition and Processing
4.2. Exploration and Identification of Key Genes
4.3. Cell Experiments
4.4. Detection of Inflammatory Cytokines and Oxidative Stress Related Factors
4.5. Transmission Electron Microscopy
4.6. Western Blotting
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | Compounds | Molecular | Classification |
---|---|---|---|
1 | Quinic acid | C7H12O6 | Phenolic acid |
2 | Chlorogenic acid | C16H18O9 | Phenolic acid |
3 | Apigenin 6-C-glucoside 8-C-arabinoside | C26H28O14 | Flavanone glycosides |
4 | Luteolin-7-O-rutinoside | C27H30O15 | Flavanone glycosides |
5 | Eriodictyol-7-O-glucoside | C21H22O11 | Flavanone glycosides |
6 | Luteolin-7-O-glucoside | C21H20O11 | Flavanone glycosides |
7 | Apigenin-7-O-glucoside | C21H20O10 | Flavanone glycosides |
8 | Rosmarinic acid | C18H16O8 | Phenolic acid |
9 | (2R)Eriodictyol 7-O-(6″-3,4-dihydroxycinnamoyl)-β-D-glucopyranoside | C30H28O14 | Flavanone glycosides |
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Sun, J.; Jiang, X.; Chen, Y.; Guo, S.; Zhao, Z.; Cao, J.; Liu, Y.; Cheng, G.; Li, Y.; Tian, L. Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses. Int. J. Mol. Sci. 2022, 23, 15651. https://doi.org/10.3390/ijms232415651
Sun J, Jiang X, Chen Y, Guo S, Zhao Z, Cao J, Liu Y, Cheng G, Li Y, Tian L. Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses. International Journal of Molecular Sciences. 2022; 23(24):15651. https://doi.org/10.3390/ijms232415651
Chicago/Turabian StyleSun, Jin, Xiaoqian Jiang, Yuxu Chen, Shancheng Guo, Zhiye Zhao, Jianxin Cao, Yaping Liu, Guiguang Cheng, Ye Li, and Lei Tian. 2022. "Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses" International Journal of Molecular Sciences 23, no. 24: 15651. https://doi.org/10.3390/ijms232415651