Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms
Abstract
:1. Introduction
2. Results
2.1. MIC Determination
2.2. Biofilm Eradication by Phages
2.3. Biofilm Eradication by Antibiotics
2.4. SEM Analysis of Biofilm
2.5. Galleria mellonella Survival Assay
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. Phages
4.3. MIC Determination
4.4. Biofilm Eradication
4.5. Microscopic Examination of Biofilms
4.6. Phages and Antibiotics Effectiveness in an In Vivo Model—Galleria mellonella
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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MDRSA Strain | MIC Value [µg × mL−1] | ||||
---|---|---|---|---|---|
SXT | GM | TE | FA | VAN | |
44 | 2.048 | 0.38 | R | 0.188 | 1.5 |
70 | 1.024 | 0.38 | R | 0.188 | 1.5 |
110 | 2.048 | 0.25 | 0.094 | 0.125 | 1.5 |
113 | 2.048 | 0.19 | 0.094 | 0.188 | 1 |
115 | 4.096 | 0.25 | 0.094 | 0.094 | 1.5 |
124 | 2.048 | 0.19 | 0.094 | 0.125 | 1 |
203 | 2.048 | 0.19 | 0.094 | 0.125 | 1.5 |
316 | 2.048 | 0.76 | 0.375 | 0.375 | 1.5 |
317 | 2.048 | 0.76 | 0.375 | 0.188 | 1.5 |
352 | 2.048 | 0.38 | 0.375 | 0.188 | 1.5 |
370 | 2.048 | 0.38 | 0.094 | 0.094 | 1.5 |
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Kaźmierczak, N.; Grygorcewicz, B.; Roszak, M.; Bochentyn, B.; Piechowicz, L. Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms. Int. J. Mol. Sci. 2022, 23, 1274. https://doi.org/10.3390/ijms23031274
Kaźmierczak N, Grygorcewicz B, Roszak M, Bochentyn B, Piechowicz L. Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms. International Journal of Molecular Sciences. 2022; 23(3):1274. https://doi.org/10.3390/ijms23031274
Chicago/Turabian StyleKaźmierczak, Natalia, Bartłomiej Grygorcewicz, Marta Roszak, Beata Bochentyn, and Lidia Piechowicz. 2022. "Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms" International Journal of Molecular Sciences 23, no. 3: 1274. https://doi.org/10.3390/ijms23031274