Glutaredoxin Interacts with GR and AhpC to Enhance Low-Temperature Tolerance of Antarctic Psychrophile Psychrobacter sp. ANT206
Abstract
:1. Introduction
2. Results and Discussion
2.1. Construction and Analysis of the Deletion Mutant Δpsgrx
2.2. PsGrx Positively Regulates the Response to Low Temperature
2.3. Screening for Proteins That Interact with PsGrx
2.4. PsGrx Interacts with GR and AhpC
2.5. PsGrx Is Participated in Glutathione Metabolism by Enhancing GR Activity
2.6. PsGrx Is Involved in ROS-Elimination Pathway by Regulating AhpC
3. Conclusions
4. Materials and Methods
4.1. Strains and Material
4.2. Construction of Mutant Strain Δpsgrx, Δahpc and Δpsgrx Δahpc
4.3. Low-Temperature Treatment, MDA Activity and Protein Carbonylation Assay
4.4. cDNA Library of Strain ANT206
4.5. Yeast Two-Hybrid Analysis
4.6. Bimolecular Fluorescence Complementation (BiFC) and Co-Immunoprecipitation (Co-IP) Assay
4.7. RNA Extraction and Quantitative Real-Time PCR
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Number | Gene Name | Functional Class |
---|---|---|
1 | Glutathione reductase | Stress-related reactions |
2 | Glutathione peroxidase | Stress-related reactions |
3 | Alkyl hydroperoxide reductase | Stress-related reactions |
4 | DNA photolyase | DNA modification |
5 | Aldehyde dehydrogenase | Biosynthesis |
6 | Translation elongation factor | Translation |
7 | Nucleoside diphosphate kinase | Translation |
8 | Glyceraldehyde 3-phosphate dehydrogenase | Glycolysis |
9 | Transketolase | Calvin cycle; associated reactions |
10 | Fructose-1,6-bisphosphatase | Calvin cycle; associated reactions |
11 | Triosephosphate isomerase | Calvin cycle; associated reactions |
12 | Fructose-bisphosphate aldolase | Calvin cycle; associated reactions |
13 | Phosphoglycerate kinase | Calvin cycle; associated reactions |
14 | Carbonic anhydrase | Calvin cycle; associated reactions |
15 | Methionine synthase | Sulfur metabolism |
16 | Cysteine synthase | Sulfur metabolism |
17 | Aminotransferase | Nitrogen metabolism |
18 | GspI | Protein secretion |
19 | DNA translocase FtsK | DNA transportation; cell division |
20 | RNase E | RNA metabolism; transcription |
21 | AarF/Abc1/UbiB kinase family protein | Fatty acid metabolism; protein synthesis |
22 | peptidoglycan-binding protein LysM | Cell separation |
23 | ATP synthase α chain | ATP metabolism |
24 | Methyltransferase small domain | Hypothetical protein |
25 | Thioesterase | Hypothetical protein |
26 | Transglutaminase-like domain | Hypothetical protein |
Name | Primer Sequences | Restriction Enzyme Cutting Sites |
---|---|---|
Δpsgrx-P1 | 5′-GCTCTAGACGATGACTGTATCTGTTAAAG-3′ | XbaI |
Δpsgrx-P2 | 5′-CGGTGCGATAGTTATTCTCTTCATAATC-3′ | |
Δpsgrx-P3 | 5′-GAAGAGAATAACTATCGCACCGTGC-3′ | |
Δpsgrx-P4 | 5′-TAGAGCTCTTAACCCGCTAATAGCTC-3′ | SacI |
Δahpc-P1 | 5′-ATGTCTAGAATGACGACTGATAGCG-3′ | XbaI |
Δahpc-P2 | 5′-GTTCTCAATGTGACCCCAAAAATAG-3′ | |
Δahpc-P3 | 5′-CAAGAGTTACACAGATAAAAACCCC-3′ | |
Δahpc-P4 | 5′-TCAGAGCTCTAAAAACTGACGACAG-3′ | SacI |
Name | Primer Sequences |
---|---|
psgrx-F | 5′-ACAAGTTTGTACAAAAAAATGACTGTATCTGTTAAAGTTTATAC-3′ |
psgrx-R | 5′-CACCACTTTGTACAAGAAACCCGCTAATAGCTCGTCAAG-3′ |
gr-F | 5′-ATGACAAAACATTATGATTATATTT TCCATTGGCGGC-3′ |
gr-R | 5′-CTAACGCATCGTCACAAACTCTTCTGAGCCAGTTGGATGAAT-3′ |
ahpc-F | 5′-ATGACGACTGATAGCGACAAGACGACTGAGAGATCTAAAAAG-3′ |
ahpc-R | 5′-AAAAACTGACGACAGCCACAATCTTAATTTCAATGACCATAAC-3′ |
glutathione peroxidase-F | 5′-ATGACTACTATTTATGATTTTAGTGCTGAGCGTATGGCAT-3′ |
glutathione peroxidase-R | 5′-TTTGCACGCCTCCTTAACTTGGTCAAGATCAGGGCTGAAC-3′ |
gspI-F | 5′-ATGATAAATAATGACAGAGCCAAACCTAACCATGTAAACCGA-3′ |
gspI-R | 5′-GTTTGGCTCTGTCATTATTTATCATTTCGGTTTACATGGTTAG-3′ |
ftsK-F | 5′-GTGATATCAGCACCAATTATTGATTACTTAAAAAAGGGCATA-3′ |
ftsK-R | 5′-AATCAATAATTGGTGCTGATATCACATATGCCCTTT-3′ |
Rnase E-F | 5′-ATGAAACGCATTTTAATCAACGCCACCCAAAACGAAGAAATTC-3′ |
Rnase E-R | 5′-GCTCTCTCTATCTGAGTTATCTGAATCATCTGACTCTAGTT-3′ |
Name | Primer Sequences |
---|---|
psgrx-F | 5′-GGCGTTGATTATGAAGAGATTGGCATG-3′ |
psgrx-R | 5′-TGTGGCACGGTACGATAGTTATTAGTC-3′ |
gr-F | 5′-TGTATGTCCGTCAGCACTCG-3′ |
gr-R | 5′-TCGCCCAAATCAAGCAGTCT-3′ |
ahpc-F | 5′-CAAGTCCGGCTCTGACCAAG-3′ |
ahpc-R | 5′-CTTGGCTCATCTCGCCATCT-3′ |
trxR-F | 5′-CTGATCGTCAACAGCGGTCT-3′ |
trxR-R | 5′-CAGCAGAGGTGATCGCTTGA-3′ |
16S-F | 5′-CCTTCGCCATCGGTATTCCTCCAG-3′ |
16S-R | 5′-GAGCTAGAGTATGTGAGAGG-3′ |
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Wang, Y.; Wang, Q.; Hou, Y.; Liu, J. Glutaredoxin Interacts with GR and AhpC to Enhance Low-Temperature Tolerance of Antarctic Psychrophile Psychrobacter sp. ANT206. Int. J. Mol. Sci. 2022, 23, 1313. https://doi.org/10.3390/ijms23031313
Wang Y, Wang Q, Hou Y, Liu J. Glutaredoxin Interacts with GR and AhpC to Enhance Low-Temperature Tolerance of Antarctic Psychrophile Psychrobacter sp. ANT206. International Journal of Molecular Sciences. 2022; 23(3):1313. https://doi.org/10.3390/ijms23031313
Chicago/Turabian StyleWang, Yatong, Quanfu Wang, Yanhua Hou, and Jianan Liu. 2022. "Glutaredoxin Interacts with GR and AhpC to Enhance Low-Temperature Tolerance of Antarctic Psychrophile Psychrobacter sp. ANT206" International Journal of Molecular Sciences 23, no. 3: 1313. https://doi.org/10.3390/ijms23031313