Peer-Review Record
Multidimensional Functional Profiling of Human Neuropathogenic FOXG1 Alleles in Primary Cultures of Murine Pallial Precursors
Int. J. Mol. Sci. 2022, 23(3), 1343; https://doi.org/10.3390/ijms23031343
by
Simone Frisari 1
, Manuela Santo 1,†,‡, Ali Hosseini 2,†, Matteo Manzati 2,†, Michele Giugliano 2 and Antonello Mallamaci 1,*
, Manuela Santo 1,†,‡, Ali Hosseini 2,†, Matteo Manzati 2,†, Michele Giugliano 2 and Antonello Mallamaci 1,*Reviewer 1:
Anonymous
Reviewer 2:
Anonymous
Int. J. Mol. Sci. 2022, 23(3), 1343; https://doi.org/10.3390/ijms23031343
Submission received: 30 December 2021
/
Revised: 20 January 2022
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Accepted: 21 January 2022
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Published: 25 January 2022
(This article belongs to the Special Issue Rare Monogenic Diseases: Molecular Pathophysiology and Novel Therapies 2.0)
Round 1
Reviewer 1 Report
Frisari et al. present here an extensive work regarding functional profiling of three FOXG1 alleles in vitro. The manuscript is very well written and the work greatly presented. I have just few suggestions for the authors.
- Despite IJMS does not put limits on articles' length, this manuscript results very long and not very reader-friendly. The authors should consider to move part of the manuscript to the Supporting Information (SI). By the way, I suggest the authors to produce a proper SI file with all the figures and the related captions.
- In the Results section, the authors could make the text more readable by removing and distributing the statistical analysis in the caption of related figures. Also, "decrease by -XX%" is not correct, please remove the minus sign and correct it to "decrease by XX%"
- The choice of those three particular alleles is not very clear. Please, briefly add an explanation in the Introduction section.
- Some abbreviations are not properly explicated, e.g. ASD-like, LOF, GOF, Plap.
- Some references are not correctly reported in the text, especially in the Discussion and Conclusions section. Please, correct.
- I would suggest the authors to split the Discussion and Conclusions into two separated sections.
- Lines 127- 141 are not justified neither present proper margins.
Finally, I would like to express my compliments to the authors for this very interesting work.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript by Frisari et al., presents data on FOXG1 alleles in primary cultures. the experiments are well described and elegant. I have few minor comments:
- in the discussion it is stated "Remarkably, our study was performed in wild-type neocortical precursors, engineered to overexpress exogenous Foxg1 alleles and, therefore, characterized bycumulative Foxg1-mRNA levels well above the physiological baseline". the authors further discuss this point, but I was wondering if it could be possible to knockdown endogenous levels before lentiviral transfection, that would also better model heterozygous mutations?
- in the intriduction, please revise gene-protein nomenclature
- Figure 5: it is very difficult to read headings, please improve.
- in the abstract, at the end it is written :"namely a step
propedeutic to timely delivery of precision treatments in utero". this sentence should be removed or modified. in utero treatments are far to be envisaged in this context and I agree that this manuscript increases the information towards personalized medicine, but not for "in utero treatments".
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
