Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell–Trophoblast Interactions
Abstract
:1. Introduction
2. Results
2.1. Influence of Cytokines on the Phenotype of the NK-92 Cell Line in Monoculture and under Co-Cultivation Conditions with the JEG-3 Cell Line
2.1.1. Effect of TNFα on the Phenotype of the NK-92 Cell Line in Monoculture and under Co-Cultivation Conditions with the JEG-3 Cell Line
2.1.2. Effect of IL-10 on the Phenotype of the NK-92 Cell Line in Monoculture and under Co-Cultivation Conditions with the JEG-3 Cell Line
2.1.3. Influence of IFNγ on the Phenotype of the NK-92 Cell Line in Monoculture and under Co-Cultivation Conditions with the JEG-3 Cell Line
2.1.4. Effect of TGFβ on the Phenotype of the NK-92 Cell Line in Monoculture and under Co-Cultivation Conditions with the JEG-3 Cell Line
2.2. Cytotoxicity of NK-92 Cell Line after Culture with Cytokines
2.3. Phenotype of Peripheral Blood NK Cells in Monoculture and under Co-Cultivation Conditions with the JEG-3 Cell Line
3. Discussion
4. Materials and Methods
4.1. Cell Cultures
4.2. Peripheral Blood Mononuclear Cells
4.3. Cytokines
4.4. Assessment of the Phenotype of the NK-92 Cell Line after Cultivation with JEG-3 Trophoblast Cells and Cytokines
4.5. Evaluation of the Cytotoxicity of the NK-92 Cell Line after Pre-Cultivation with Cytokines
4.6. Evaluation of the Phenotype of pNK Cells after Cultivation with JEG-3 Trophoblast Cells and Cytokines
4.7. Statistical Data Analysis
4.8. Ethical Approval
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Mikhailova, V.; Grebenkina, P.; Khokhlova, E.; Davydova, A.; Salloum, Z.; Tyshchuk, E.; Zagainova, V.; Markova, K.; Kogan, I.; Selkov, S.; et al. Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell–Trophoblast Interactions. Int. J. Mol. Sci. 2022, 23, 2387. https://doi.org/10.3390/ijms23042387
Mikhailova V, Grebenkina P, Khokhlova E, Davydova A, Salloum Z, Tyshchuk E, Zagainova V, Markova K, Kogan I, Selkov S, et al. Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell–Trophoblast Interactions. International Journal of Molecular Sciences. 2022; 23(4):2387. https://doi.org/10.3390/ijms23042387
Chicago/Turabian StyleMikhailova, Valentina, Polina Grebenkina, Evgeniia Khokhlova, Alina Davydova, Zeina Salloum, Elizaveta Tyshchuk, Valeria Zagainova, Kseniia Markova, Igor Kogan, Sergey Selkov, and et al. 2022. "Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell–Trophoblast Interactions" International Journal of Molecular Sciences 23, no. 4: 2387. https://doi.org/10.3390/ijms23042387