Thermally Stable and Reusable Ceramic Encapsulated and Cross-Linked CalB Enzyme Particles for Rapid Hydrolysis and Esterification
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation and Characterization of Si-E-CPs and Si-CL-CPs
2.2. Thermal Stability, pH Activity, and Reusability of Si-E-CPs and Si-CL-CPs
2.3. Enzymatic Hydrolysis for p-Nitrophenyl Butyrate
2.4. Enzymatic Esterification of Benzyl Benzoate with Si-CL-CPs
2.5. Conversion of Benzyl Benzoate from Benzoic Anhydride with Native CalB and Si-CL-CPs
3. Materials and Methods
3.1. Materials
3.2. Preparation of Si-E-CPs and Si-CL-CPs
3.3. Determination of CalB Enzyme Amounts in Si-E-CPs and Si-CL-CPs
3.4. Thermal Stability and pH Activity of Native CalB, Si-E-CPs, and Si-CL-CPs
3.5. Reusability of Si-E-CPs and Si-CL-CPs
3.6. Enzymatic Hydrolysis for p-Nitrophenyl Butyrate
3.7. Enzymatic Synthesis for Benzyl Benzoate with Native CalB and Si-CL-CPs
3.8. Instrumental Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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[TEOS] (mM) | Km (μM) | Vmax (μM ⸱ min−1) | Kcat (min−1) | Kcat/Km (mM−1 ⸱ min−1) | |
---|---|---|---|---|---|
Native CalB | 0 | 432.60 | 16.86 | 15.03 | 34.76 |
Si−E−CPs | 10 | 915.32 | 4.57 | 5.03 | 5.50 |
50 | 588.31 | 9.94 | 9.90 | 16.83 | |
Si−CL−CPs | 100 | 517.31 | 12.02 | 10.70 | 20.68 |
300 | 497.86 | 12.06 | 10.73 | 21.56 |
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Song, M.; Chang, J.-H. Thermally Stable and Reusable Ceramic Encapsulated and Cross-Linked CalB Enzyme Particles for Rapid Hydrolysis and Esterification. Int. J. Mol. Sci. 2022, 23, 2459. https://doi.org/10.3390/ijms23052459
Song M, Chang J-H. Thermally Stable and Reusable Ceramic Encapsulated and Cross-Linked CalB Enzyme Particles for Rapid Hydrolysis and Esterification. International Journal of Molecular Sciences. 2022; 23(5):2459. https://doi.org/10.3390/ijms23052459
Chicago/Turabian StyleSong, Min, and Jeong-Ho Chang. 2022. "Thermally Stable and Reusable Ceramic Encapsulated and Cross-Linked CalB Enzyme Particles for Rapid Hydrolysis and Esterification" International Journal of Molecular Sciences 23, no. 5: 2459. https://doi.org/10.3390/ijms23052459