Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Composite Components
4.2. Composite Sheets Fabrication
4.3. HBMSC Isolation and Culture Expansion
4.4. Cultures in the Presence of CMs Harvested from the Materials
4.5. Migration Studies
4.6. Metabolic Activity of Cells
4.7. Gene Expression Analyses
4.8. Western Blot Analyses
4.9. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Material | Ions Concentration [mM/dm3] | ||
---|---|---|---|
Calcium Ions (Ca2+) | Phosphate Ions (PO43−) | Silicate Ions (SiO44−) | |
A1/PLGA | 10.835 ± 0.047 | 0.637 ± 0.016 | 1.372 ± 0.030 |
A2/PLGA | 8.404 ± 0.075 | 0.707± 0.013 | 1.835 ± 0.093 |
S1/PLGA | 5.510 ± 0.025 | 1.180± 0.034 | 0.631 ± 0.006 |
S2/PLGA | 2.465 ± 0.049 | 1.164 ± 0.030 | 1.205 ± 0.018 |
PLGA | 0.674 ± 0.020 | 1.366 ± 0.046 | 0.002 ± 3.746 × 10−4 |
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Łukowicz, K.; Zagrajczuk, B.; Truchan, K.; Niedzwiedzki, Ł.; Cholewa-Kowalska, K.; Osyczka, A.M. Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration. Int. J. Mol. Sci. 2022, 23, 2598. https://doi.org/10.3390/ijms23052598
Łukowicz K, Zagrajczuk B, Truchan K, Niedzwiedzki Ł, Cholewa-Kowalska K, Osyczka AM. Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration. International Journal of Molecular Sciences. 2022; 23(5):2598. https://doi.org/10.3390/ijms23052598
Chicago/Turabian StyleŁukowicz, Krzysztof, Barbara Zagrajczuk, Karolina Truchan, Łukasz Niedzwiedzki, Katarzyna Cholewa-Kowalska, and Anna M. Osyczka. 2022. "Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration" International Journal of Molecular Sciences 23, no. 5: 2598. https://doi.org/10.3390/ijms23052598