Molecular Characterisation of Flavanone O-methylation in Eucalyptus
Abstract
:1. Introduction
2. Results
2.1. Flavanone Profiling of Various Eucalyptus Species
2.2. Isolation of O-methyltransferase from E. nitida
2.3. Kinetic Analysis of EnOMT1
2.4. Isolation of OMT1 in Other Eucalyptus Species
2.5. Gene Expression of OMT1 in E. nitida and E. mitchelliana
2.6. Activity of OMT1 Homologues in Additional Eucalyptus Species
2.7. Analysis of Critical Residues of EnOMT1
3. Discussion
4. Materials and Methods
4.1. Plant Material and Flavanone Extraction
4.2. RNA Isolation
4.3. Transcriptome Sequencing
4.4. Recombinant Protein Expression
4.5. Site-Directed Mutagenesis
4.6. Enzyme Assay and Kinetics
4.7. Liquid Chromatography and Mass Spectrometry of Flavanones
4.8. Quantitative Real-Time PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Somaletha Chandran, K.; Humphries, J.; Goodger, J.Q.D.; Woodrow, I.E. Molecular Characterisation of Flavanone O-methylation in Eucalyptus. Int. J. Mol. Sci. 2022, 23, 3190. https://doi.org/10.3390/ijms23063190
Somaletha Chandran K, Humphries J, Goodger JQD, Woodrow IE. Molecular Characterisation of Flavanone O-methylation in Eucalyptus. International Journal of Molecular Sciences. 2022; 23(6):3190. https://doi.org/10.3390/ijms23063190
Chicago/Turabian StyleSomaletha Chandran, Krishna, John Humphries, Jason Q.D. Goodger, and Ian E. Woodrow. 2022. "Molecular Characterisation of Flavanone O-methylation in Eucalyptus" International Journal of Molecular Sciences 23, no. 6: 3190. https://doi.org/10.3390/ijms23063190