Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants
Abstract
:1. Introduction
2. Results
2.1. Identification and Bioinformatics Analysis of the PtDi19 Genes
2.2. Analysis of Evolutionary Trees, Conserved Protein Domain, motifs, and Gene Structure
2.3. Expression Profiles of PtDi19 Genes under Different Stresses
2.4. Subcellular Localization and Transcriptional Activity
2.5. PtDi19-2 Interactions with PtDi19-7 as Co-transcription Factors
2.6. Transgenic Arabidopsis Improved Sensitivity to ABA and Enhanced Drought Tolerance during the Germination Period
2.7. Overexpression of PtDi19-2 and PtDi19-7 Could Enhance Drought Tolerance of Transgenic Arabidopsis
2.8. PtDi19-7 Overexpression Could Enhance Drought Tolerance of Transgenic 84K Poplar
2.9. ABA-Induced Lateral Bud Dormancy in 84K Poplar
2.10. Overexpression of PtDi19-2 and PtDi19-7 Promoted ABA-Induced Stomatal Closure and Upregulated Expression of Stress-Response Genes
3. Discussion
4. Materials and Methods
4.1. Identification and Bioinformatics Analysis of Di19 Gene Family
4.2. Phylogenetic Tree, Gene Structure, and Conserved Motif Analysis
4.3. Transcriptome Data to Analyze the Expression Patterns of PtDi19
4.4. Plant Materials, Treatments, and RNA Extraction
4.5. Quantitative Real-Time PCR Analysis
4.6. Subcellular Localization and Transactivation Activity
4.7. Yeast Two-Hybridization
4.8. Phenotypic Analysis of Transgenic Plants
4.9. ABA Sensitivity Test
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
Di19 | Drought-induced 19 |
ABA | Abscisic acid |
MDA | Malondialdehyde |
POD | Peroxidase |
DAB | Diaminobenzidine |
PEG | Polyethylene glycol |
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Wu, C.; Lin, M.; Chen, F.; Chen, J.; Liu, S.; Yan, H.; Xiang, Y. Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants. Int. J. Mol. Sci. 2022, 23, 3371. https://doi.org/10.3390/ijms23063371
Wu C, Lin M, Chen F, Chen J, Liu S, Yan H, Xiang Y. Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants. International Journal of Molecular Sciences. 2022; 23(6):3371. https://doi.org/10.3390/ijms23063371
Chicago/Turabian StyleWu, Caijuan, Miao Lin, Feng Chen, Jun Chen, Shifan Liu, Hanwei Yan, and Yan Xiang. 2022. "Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants" International Journal of Molecular Sciences 23, no. 6: 3371. https://doi.org/10.3390/ijms23063371