Cleavage of Early Mouse Embryo with Damaged DNA
Abstract
:1. Introduction
2. Results
2.1. Incorporation of EdU into DNA in Individual Cell Cycle Phases of One-Cell Embryos
2.2. Neocarzinostatin Increasesthe Incidence of yH2AXSer139-Positive Foci during the First Embryonic Cell Cycle
2.3. DNA Damage Reduce the Cleavage Ability of Early Embryos
2.4. Higher DNA Damage Level Cause Decreased Volume of DNA after the End of the S-Phase
2.5. DNA Damage CanInduce Chromosomal Missegregation
2.6. Low Dose of NCS Does Not Affect Entry into Anaphase but Induces Micronuclei Formation
2.7. DNA Damage Increases Chromatin Fragmentation and Reduces the Number of Blastomeres
3. Discussion
4. Materials and Methods
4.1. Embryo Collection and In Vitro Culture
4.2. Evaluation of the Ability to Cleavage
4.3. Immunocytochemistry
4.4. Propidium Staining
4.5. Time-Lapse Recording
4.6. TUNEL Assay
4.7. Image Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
APC/C | Anaphase-promoting complex/cyclosome |
DAPI | 4’,6-diamidino-2-phenylindole |
DSBs | Double-stranded breaks |
eCG | Pregnant mare’s serum gonadotropin |
EdU | 5-ethynyl-2’-deoxyuridine |
EGFP | Enhanced green fluorescent protein |
FITC | Fluorescein.5-isothiocyanate |
hCG | Human chorionic gonadotropin |
MN/MNs | Micronuleus/Micronuclei |
NCS | Neocarzinostatin |
NEBD | Nuclear envelope breakdown |
PBS | Phosphate buffer saline |
RT | Room temperature |
SAC | Spindle assembly checkpoint |
TRITC | Tetramethylrhodamine |
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Baran, V.; Pisko, J. Cleavage of Early Mouse Embryo with Damaged DNA. Int. J. Mol. Sci. 2022, 23, 3516. https://doi.org/10.3390/ijms23073516
Baran V, Pisko J. Cleavage of Early Mouse Embryo with Damaged DNA. International Journal of Molecular Sciences. 2022; 23(7):3516. https://doi.org/10.3390/ijms23073516
Chicago/Turabian StyleBaran, Vladimír, and Jozef Pisko. 2022. "Cleavage of Early Mouse Embryo with Damaged DNA" International Journal of Molecular Sciences 23, no. 7: 3516. https://doi.org/10.3390/ijms23073516