Profiles of Cytokinins Metabolic Genes and Endogenous Cytokinins Dynamics during Shoot Multiplication In Vitro of Phalaenopsis
Abstract
:1. Introduction
2. Results
2.1. Identification of IPT, LOG, and CKX Proteins in Phalaenopsis
2.2. Expression of IPT, LOG, and CKX Genes in Different Organs
2.3. Effect of TDZ Treatments on Shoot Multiplication In Vitro
2.4. Expression of IPT, LOG, and CKX Genes in Micropropagated Shoots
2.5. Effect of TDZ Treatments on Endogenous Cytokinin-Ribosides Levels
2.6. Transient Overexpression of PaIPT1, PaLOG2, and PaCKX4 in Tobacco Leaves
3. Discussion
4. Materials and Methods
4.1. Identification of Phalaenopsis IPT, LOG, and CKX Genes
4.2. Plant Materials and Tissue Culture Experiments
4.3. Quantitative RT-PCR (RT-qPCR)
4.4. Transient Overexpression of PaIPT, PaLOG, and PaCKX Proteins in Tobacco Leaves
4.5. Quantification of Endogenous CK-Ribosides
4.6. Experimental Design and Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, Y.-Y.; Hao, Z.-G.; Miao, S.; Zhang, X.; Li, J.-Q.; Guo, S.-X.; Lee, Y.-I. Profiles of Cytokinins Metabolic Genes and Endogenous Cytokinins Dynamics during Shoot Multiplication In Vitro of Phalaenopsis. Int. J. Mol. Sci. 2022, 23, 3755. https://doi.org/10.3390/ijms23073755
Li Y-Y, Hao Z-G, Miao S, Zhang X, Li J-Q, Guo S-X, Lee Y-I. Profiles of Cytokinins Metabolic Genes and Endogenous Cytokinins Dynamics during Shoot Multiplication In Vitro of Phalaenopsis. International Journal of Molecular Sciences. 2022; 23(7):3755. https://doi.org/10.3390/ijms23073755
Chicago/Turabian StyleLi, Yuan-Yuan, Zhi-Gang Hao, Shuo Miao, Xiong Zhang, Jian-Qiang Li, Shun-Xing Guo, and Yung-I Lee. 2022. "Profiles of Cytokinins Metabolic Genes and Endogenous Cytokinins Dynamics during Shoot Multiplication In Vitro of Phalaenopsis" International Journal of Molecular Sciences 23, no. 7: 3755. https://doi.org/10.3390/ijms23073755