Transcriptional Regulation of RIP2 Gene by NFIB Is Associated with Cellular Immune and Inflammatory Response to APEC Infection
Abstract
:1. Introduction
2. Results
2.1. Identification of NOD-like Receptor Signaling Pathway and RIP2 as APEC Regulator
2.2. APEC Increased RIP2 Expression and Suppressed Chicken HD11 Cells Viability
2.3. RIP2 Knockdown Rescued the APEC-Stimulated Effect on Viability and Apoptosis of Chicken HD11 Cells
2.4. Identification of the Chicken RIP2 Promoter Region and Analysis of Transcriptional Regulatory Elements
2.5. Identification of Transcription Factors in Core Region of Chicken RIP2 Promoter
2.6. Design and Screening of siRNA Interfering with NFIB
2.7. RIP2 Was Positively Regulated by NFIB Expression upon APEC Infection
2.8. NFIB Exacerbated APEC-Induced Injuries through Modulation of RIP2 in Chicken HD11 Cells
2.9. NFIB Overexpression Activated NFκB via Up-Regulating RIP2
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. RT-qPCR
4.3. Apoptosis Assay
4.4. Cell Viability Assay
4.5. Cell Transfection and Dual-Luciferase Reporter Assay for RIP2 Promoter
4.6. Bioinformatics Prediction
4.7. Construction of Plasmids
4.8. CHIP-PCR
4.9. Western Blot Analysis
4.10. Nitric Oxide Production Assay
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sun, H.; Li, N.; Tan, J.; Li, H.; Zhang, J.; Qu, L.; Lamont, S.J. Transcriptional Regulation of RIP2 Gene by NFIB Is Associated with Cellular Immune and Inflammatory Response to APEC Infection. Int. J. Mol. Sci. 2022, 23, 3814. https://doi.org/10.3390/ijms23073814
Sun H, Li N, Tan J, Li H, Zhang J, Qu L, Lamont SJ. Transcriptional Regulation of RIP2 Gene by NFIB Is Associated with Cellular Immune and Inflammatory Response to APEC Infection. International Journal of Molecular Sciences. 2022; 23(7):3814. https://doi.org/10.3390/ijms23073814
Chicago/Turabian StyleSun, Hongyan, Naying Li, Jishuang Tan, Huan Li, Jibin Zhang, Lujiang Qu, and Susan J. Lamont. 2022. "Transcriptional Regulation of RIP2 Gene by NFIB Is Associated with Cellular Immune and Inflammatory Response to APEC Infection" International Journal of Molecular Sciences 23, no. 7: 3814. https://doi.org/10.3390/ijms23073814