New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro
Abstract
:1. Introduction
2. Results and Discussions
2.1. p(NIPAM-BA) Interfaces’ Morphology Obtained by MAPLE
2.1.1. Water-Solvent
2.1.2. DMSO-Solvent
2.1.3. Chloroform-Solvent
2.2. p(NIPAM-BA) Interfaces’ Morphology Obtained by Spin Coating (SC)
2.3. FTIR Investigations of the p(NIPAM-BA) Copolymer Coatings
2.4. Surface Composition Measurements by X-Ray Photoelectron Spectroscopy (XPS) Analysis
2.5. Contact Angles Measurements and Swelling Behavior
2.6. Biological In Vitro Investigations
2.6.1. Cell Viability
2.6.2. Adhesion and Distribution of Cells onto the p(NIPAM-BA) Layers
3. Materials and Methods
3.1. Materials and Reagents
3.2. Surface Designing/Tailoring/Deposition
3.2.1. MAPLE Method
3.2.2. Spin Coating Method
3.3. p(NIPAM-BA) Morphologic Analysis
3.3.1. Scanning Electron Microscopy (SEM)
3.3.2. Atomic Force Microscopy (AFM)
3.4. Wettability Characterization by Contact Angle (CA)
3.5. Chemical Profile: Analysis of p(NIPAM-BA)
3.5.1. Fourier Transform Infrared Spectroscopy (FTIR) Analysis
3.5.2. X-ray Photoelectron Spectroscopy (XPS) Analysis
3.6. Biological Investigations
3.6.1. Cells
3.6.2. Cell Viability Assay
3.6.3. Cell Adhesion by Scanning Electron Microscopy (SEM)
3.6.4. Statistical Analysis
3.7. Samples/Batch Production
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Roughness—RMS (nm) | ||||
---|---|---|---|---|
Deposition Methods | p(NIPAM-BA) 2 wt.%+ H2O | p(NIPAM-BA) 2 wt.%+ DMSO | p(NIPAM-BA) 2 wt.%+ Chloroform | |
Drop-cast | 4.4 | 0.8 | 2.8 | |
MAPLE | 250 mJ/cm2 | 36.3 | 1.5 | 25.7 |
350 mJ/cm2 | 139 | 27.7 | 143 | |
450 mJ/cm2 | 346 | 49.4 | 153.2 | |
Spin Coating | 3.6 | 7 | 0.9 |
Solvent Used | Deposition Technique | |||||
---|---|---|---|---|---|---|
Drop-Cast | MAPLE Laser Fluence | Spin Coating | Atomic Composition (%) | |||
250 mJ/cm2 | 350 mJ/cm2 | 450 mJ/cm2 | ||||
Water | 73.48 | 54.54 | 69.73 | 72.10 | 73.23 | C1s |
12.01 | 9.66 | 13.19 | 13.18 | 13.03 | N1s | |
14.51 | 26.79 | 17.08 | 14.71 | 13.74 | O1s | |
- | 9.01 | - | - | - | Si2p | |
DMSO | 73.72 | 55.96 | 64.57 | 64.00 | 72.99 | C1s |
12.30 | 9.91 | 11.76 | 11.17 | 13.57 | N1s | |
13.98 | 27.34 | 20.30 | 20.77 | 13.44 | O1s | |
- | 6.79 | 3.38 | 4.06 | - | S2p | |
Chlorofom | 72.71 | 59.83 | 67.83 | 66.16 | 74.15 | C1s |
12.82 | 5.62 | 10.70 | 9.23 | 12.78 | N1s | |
14.47 | 18.04 | 16.63 | 17.11 | 13.07 | O1s | |
- | 16.51 | 4.84 | 7.50 | - | Cl2p |
p(NIPAM-BA) _Water | ||
---|---|---|
Laser Fluence (mJ/cm2) | CA (RT) CA (37 °C) | |
Drop-Cast | 63.73 ± 0.34 | 70.87 ± 0.72 |
250 | 25.85 ± 0.52 | 28.24 ± 0.5 |
350 | 28.99 ± 0.13 | 33.4 ± 0.22 |
450 | 36.75 ± 0.41 | 45.07 ± 0.37 |
p(NIPAM-BA) _DMSO | ||
---|---|---|
Laser Fluence (mJ/cm2) | CA (RT) CA (37 °C) | |
Drop-Cast | 75.79 ± 1.24 | 60 ± 0.76 |
250 | 56.47 ± 0.84 | 62.31 ± 0.46 |
350 | 78.49 ± 0.58 | 75.7 ± 0.66 |
450 | 84.14 ± 1.36 | 61.64 ± 0.89 |
p(NIPAM-BA) _ Chloroform | ||
---|---|---|
Laser Fluence (mJ/cm2) | CA (RT) CA (37 °C) | |
Drop-Cast | 92.58 ± 1.89 | 70.37 ± 0.79 |
250 | 91.09 ± 1.65 | 99.44 ± 1.53 |
350 | 77.44 ± 0.68 | 74.3 ± 0.85 |
450 | 77.89 ± 0.66 | 75.43 ± 0.98 |
p(NIPAM-BA) | CA (RT) | CA (37 °C) |
---|---|---|
Water | 63.73 ± 0.99 | 65.36 ± 0.36 |
DMSO | 59.2 ± 0.24 | 66.78 ± 0.15 |
Chloroform | 66.77 ± 1.166 68.77 ± 1.16 | 69.18 ± 0.72 |
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Dumitrescu, N.-L.; Icriverzi, M.; Bonciu, A.; Florian, P.; Moldovan, A.; Roseanu, A.; Rusen, L.; Dinca, V.; Grama, F. New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro. Int. J. Mol. Sci. 2022, 23, 3988. https://doi.org/10.3390/ijms23073988
Dumitrescu N-L, Icriverzi M, Bonciu A, Florian P, Moldovan A, Roseanu A, Rusen L, Dinca V, Grama F. New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro. International Journal of Molecular Sciences. 2022; 23(7):3988. https://doi.org/10.3390/ijms23073988
Chicago/Turabian StyleDumitrescu, Nicoleta-Luminita, Madalina Icriverzi, Anca Bonciu, Paula Florian, Antoniu Moldovan, Anca Roseanu, Laurentiu Rusen, Valentina Dinca, and Florin Grama. 2022. "New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro" International Journal of Molecular Sciences 23, no. 7: 3988. https://doi.org/10.3390/ijms23073988