MdMAPKKK1 Regulates Apple Resistance to Botryosphaeria dothidea by Interacting with MdBSK1
Abstract
:1. Introduction
2. Results
2.1. Apple MAPK Cascade Is Involved in the Defense against B. dothidea
2.2. MdMAPKKK1 Overexpression Leads to Death of Plant Cell
2.3. MdMAPKKK1 Silencing Impairs Apple Resistance to B. dothidea
2.4. MdMAPKKK1 Interacts with BSK1-1 and BSK1-2
2.5. BSK1 Phosphorylates MdMAPKKK1
2.6. BSK1 Silencing Decreases Apple Resistance to B. dothidea
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Pathogen
4.2. Quantitative Reverse Transcription-PCR and MAPK Activation Assay
4.3. Ectopic Transient Expression and Inducible Expression in ‘Orin’
4.4. CRISPR/cas9 Mediated Gene Mutagenesis and Virus-Induced Gene Silencing
4.5. Yeast Two Hybrid Assay
4.6. Split Luciferase Assay
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, N.; Liu, Y.; Dong, C.; Zhang, Y.; Bai, S. MdMAPKKK1 Regulates Apple Resistance to Botryosphaeria dothidea by Interacting with MdBSK1. Int. J. Mol. Sci. 2022, 23, 4415. https://doi.org/10.3390/ijms23084415
Wang N, Liu Y, Dong C, Zhang Y, Bai S. MdMAPKKK1 Regulates Apple Resistance to Botryosphaeria dothidea by Interacting with MdBSK1. International Journal of Molecular Sciences. 2022; 23(8):4415. https://doi.org/10.3390/ijms23084415
Chicago/Turabian StyleWang, Nan, Yingshuang Liu, Chaohua Dong, Yugang Zhang, and Suhua Bai. 2022. "MdMAPKKK1 Regulates Apple Resistance to Botryosphaeria dothidea by Interacting with MdBSK1" International Journal of Molecular Sciences 23, no. 8: 4415. https://doi.org/10.3390/ijms23084415