The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and Inhibits Lepidium sativum Seed Germination via Multiple Mechanisms including Interference with Auxin Homeostasis
, Matthew Walker 1, Dianne Irwin 2, Jonathan Cohn 3, Stephanie M. Guida-English 4, Lucio Garcia 3, Iva Pavlović 5, Ondřej Novák 5, Danuše Tarkowská 5, Miroslav Strnad 5, Marta Pérez 1, Anne Seville 2, David Stock 2,†and Gerhard Leubner-Metzger 1,5,*Round 1
Reviewer 1 Report
Dear Authors,
The title of the article sounds interesting. Also, performed analyses look interesting.
The article does not require significant corrections.
The introduction is comprehensive and introduces well the research topic,
In my opinion this work should make a good contribution to the literature. Further more, in my opinion, the paper will attract a wide readership. The MS looks good. Therefore, I have no serious substantive comments to the MS.
The experimentum design and data analysis are appropriate, the introduction and discussion are consistent.
Recommendations for authors:
However, the authors need to correct the technical shortcomings:
1. different font table 2, 3, 4 in comparison to table 1
2. Please read the MS once more and correct any minor shortcomings, e.g. punctuation, etc.
Author Response
We thank the reviewer for his positive comment. This reviewer had the following Recommendations for authors:
However, the authors need to correct the technical shortcomings:
1. different font table 2, 3, 4 in comparison to table 1
Response: We changed to font in tables 2,3,4 and now everything is consistent with the same font
- Please read the MS once more and correct any minor shortcomings, e.g. punctuation, etc.
Response: We read the MS once more and corrected the follewing typos:
- 7 line 238 "defence" corrected to "defense"
p.7 line 256 made A. thaliana italic
- 28 line 902 made L. sativum italic
We also added the page or article numbers to the fololwing references (they were missing): 14, 41, 43, 47, 62, 57, 73, 77, 80, 82, 86, 89, 93, 98, 102
Reviewer 2 Report
Submited paper presents results from a complex and multi-threaded experiment showing influence of allelochemical, myrigalone A (MyA) on seed germination in comparison with the influence of other bioactive and non-active compounds. Transcriptome and hormone analyses of seeds were performed. Apart from recognizing the scope of the work, good qualit of the experiment and a good way of results presentation, the reviewer has no other comments.
Author Response
We thank the reviewer for emphasising the good quality of our experiments and also our good way of presenting our results. There was no request for any revision by this reviewer.
