miR 31-3p Has the Highest Expression in Cesarean Scar Endometriosis
Abstract
:1. Introduction
2. Results
Name in DataBase | Accession Number |
hsa-miR-125b-1-3p | MIMAT0004592 |
hsa-miR-31-3p | MIMAT0004504 |
hsa-miR-1-3p | MIMAT0000123 |
hsa-miR-191-5p | MIMAT0000440 |
hsa-miR-200b-3p | MIMAT0000318 |
hsa-miR-502-5p | MIMAT0002873 |
hsa-miR-503-5p | MIMAT0002874 |
hsa-miR-548d-5p | MIMAT0004812 |
Correlations
3. Discussion
4. Materials and Methods
4.1. Tissue Preparation
4.2. RNA Extraction from FFPE Samples
4.3. Expression Profile of miRNA Genes
4.4. RT-qPCR with Individual cDNA
4.5. MicroRNA Assays
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
SE | the cesarean scar (scar) endometriosis |
DIE | deep infiltrating endometriosis |
OE | endometrioma (ovarian endometrial cyst) |
CG | control group |
FFPE | formalin-fixed paraffin-embedded tissue |
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Groups | Control Group | Ovarian Endometrial Cyst | Endometroid Ovarian Cancer | Serous HG Ovarian Cancer | |
---|---|---|---|---|---|
miRNA | |||||
miR-1-3p Assay ID: 477820_mir | 1 | 1.4029 | 1.0037 | 1.0615 | |
miR-31-3p Assay ID: 478012_mir | 1 | 0.9693 | 8.6679 | 0.9646 | |
miR-125b-1-3p Assay ID: 478665_mir | 1 | 1.2257 | 0.1475 | 0.2446 | |
miR-200b-3p Assay ID: 477963_mir | 1 | 0.2539 | 15.0699 | 7.9167 | |
miR-548d Assay ID: 480870_mir | 1 | 1.5972 | 2.1021 | 0.8198 | |
miR-502-5p Assay ID: 47954_mir | 1 | 0.1191 | 0.0055 | 0.1477 | |
miR-503-5p Assay ID: 47143_mir | 1 | 0.005 | 0.1759 | 0.5978 |
miRNA | miR31-3p | miR125b-1-3p | miR200b-3p | miR1-3p | miR502 | miR503 | miR548d | |
---|---|---|---|---|---|---|---|---|
Studied Cohort | ||||||||
Scar Endometriosis | Mean SD Median | 1.418 ± 1.570 0.961 | 0.073 ± 0.185 0.007 | 0.228 ± 0.301 0.113 | 2.925 ± 13.272 0.0004 | 2154.744 ± 5663.032 642.001 | 1.648 ± 0.916 1.768 | 3.983 ± 10.314 1.269 |
Control Group | Mean SD Median | 0.061 ± 0.176 0.001 | 0.123 ± 0.96 0.113 | 0.079 ± 0.158 0.001 | 0.03 ± 0.002 0.003 | 1142.603 ± 518.302 1014.267 | 0.075 ± 0.404 0.586 | 0.923 ± 0.431 0.832 |
Ovarian Endometrial Cyst | Mean SD Median | 0.176 ± 0.27 0.091 | 0.202 ± 0.86 0.046 | 0.06 ± 0.1 0.024 | 0.003 ± 0.01 0.002 | 3330.395 ± 13383.09 880.406 | 1.863 ± 2.16 1.1097 | 2.251 ± 6.61 0.869 |
Deep Infiltrating Endometriosis | Mean SD Median | 1.9 ± 5.44 0.099 | 1.434 ± 4.03 0.014 | 6.284 ± 20.3 0.357 | 1.069 ± 3.12 0.007 | 5347.585 ±13,997.62 369.658 | 2.058 ± 2.54 0.964 | 3900.219 ± 14,460.058 0.728 |
miRNA | DIE | SE | OE | CG | DIE | SE | OE | CG |
min | max | min | max | min | max | min | max | |
miR31-3p | 0.00060 | 20.52 | 0.00910 | 7.68 | 0.00010 | 1.59 | 0.0000 | 0.964 |
miR125b-1-3p | 0.00010 | 15.24 | 0.00010 | 0.83 | 0.00120 | 5.76 | 0.0003 | 0.429 |
miR200b-3p | 0.00120 | 76.62 | 0.00130 | 1.44 | 0.00000 | 0.58 | 0.0001 | 0.691 |
miR1-3p | 0.00000 | 11.73 | 0.00000 | 72.94 | 0.00010 | 0.03 | 0.0002 | 0.009 |
miR502 | 0.86420 | 52,830.96 | 0.48730 | 31,213.67 | 24.19780 | 91,947.80 | 311.9996 | 2351.290 |
miR503 | 0.01250 | 7.49 | 0.05290 | 3.55 | 0.01420 | 12.59 | 0.1399 | 1.742 |
miR548d | 0.01170 | 54140.58 | 0.03510 | 57.46 | 0.25490 | 44.36 | 0.0096 | 1.993 |
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Szubert, M.; Nowak-Glück, A.; Domańska-Senderowska, D.; Szymańska, B.; Sowa, P.; Rycerz, A.; Wilczyński, J.R. miR 31-3p Has the Highest Expression in Cesarean Scar Endometriosis. Int. J. Mol. Sci. 2022, 23, 4660. https://doi.org/10.3390/ijms23094660
Szubert M, Nowak-Glück A, Domańska-Senderowska D, Szymańska B, Sowa P, Rycerz A, Wilczyński JR. miR 31-3p Has the Highest Expression in Cesarean Scar Endometriosis. International Journal of Molecular Sciences. 2022; 23(9):4660. https://doi.org/10.3390/ijms23094660
Chicago/Turabian StyleSzubert, Maria, Anna Nowak-Glück, Daria Domańska-Senderowska, Bożena Szymańska, Piotr Sowa, Aleksander Rycerz, and Jacek R. Wilczyński. 2022. "miR 31-3p Has the Highest Expression in Cesarean Scar Endometriosis" International Journal of Molecular Sciences 23, no. 9: 4660. https://doi.org/10.3390/ijms23094660