Localization of BDNF and Calretinin in Olfactory Epithelium and Taste Buds of Zebrafish (Danio rerio)
Abstract
:1. Introduction
2. Results
2.1. Localization of BDNF in the Olfactory Epithelium of Zebrafish during Development
2.2. Localization of BDNF in Taste Buds and ICCC of Zebrafish during Development
3. Discussions
4. Materials and Methods
4.1. Trasmission Electron Microscopy
4.2. Immunohistochemical Detection of BDNF, Pro-BDNF, Calretinin and S100 Protein in the Olfactory Epithelium, Taste Buds and ICCC
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primary Antibodies | Supplier | Catalogue Number | Source | Diluition | Antibody ID |
---|---|---|---|---|---|
BDNF | Merck Millipore | AB1534SP | rabbit | 1:100 | AB_90748 |
pro-BDNF | Santa Cruz Biotechnology | sc-65513 | mouse | 1:100 | AB_831028 |
Calretinin | Santa Cruz Biotechnology | sc-11644 | goat | 1:100 | AB_634545 |
S100 | Dako | Z0311 | rabbit | 1:100 | AB_10013383 |
Secondary Antibodies | Supplier | Catalogue Number | Source | Diluition | Antibody ID |
---|---|---|---|---|---|
Antirabbit IgG (H+L) Alexa Fluor 594 | Invitrogen | A-21207 | donkey | 1:300 | AB_141607 |
Antirabbit IgG (H+L) Alexa Fluor 488 | Invitrogen | A-11008 | goat | 1:300 | AB_143165 |
Antigoat IgG (H+L) Alexa Fluor 594 | Invitrogen | A-11058 | donkey | 1:300 | AB_2534105 |
Antimouse IgG (H+L) Alexa Fluor 488 | Invitrogen | A-11001 | goat | 1:300 | AB_2534069 |
Antirabbit IgG-peroxidase conjugate | Amersham | NA934 | donkey | 1:100 | AB_772206 |
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Aragona, M.; Porcino, C.; Guerrera, M.C.; Montalbano, G.; Laurà, R.; Levanti, M.; Abbate, F.; Cobo, T.; Capitelli, G.; Calapai, F.; et al. Localization of BDNF and Calretinin in Olfactory Epithelium and Taste Buds of Zebrafish (Danio rerio). Int. J. Mol. Sci. 2022, 23, 4696. https://doi.org/10.3390/ijms23094696
Aragona M, Porcino C, Guerrera MC, Montalbano G, Laurà R, Levanti M, Abbate F, Cobo T, Capitelli G, Calapai F, et al. Localization of BDNF and Calretinin in Olfactory Epithelium and Taste Buds of Zebrafish (Danio rerio). International Journal of Molecular Sciences. 2022; 23(9):4696. https://doi.org/10.3390/ijms23094696
Chicago/Turabian StyleAragona, Marialuisa, Caterina Porcino, Maria Cristina Guerrera, Giuseppe Montalbano, Rosaria Laurà, Maria Levanti, Francesco Abbate, Teresa Cobo, Gabriel Capitelli, Fabrizio Calapai, and et al. 2022. "Localization of BDNF and Calretinin in Olfactory Epithelium and Taste Buds of Zebrafish (Danio rerio)" International Journal of Molecular Sciences 23, no. 9: 4696. https://doi.org/10.3390/ijms23094696