Strawberry FaSnRK1α Regulates Anaerobic Respiratory Metabolism under Waterlogging
Abstract
:1. Introduction
2. Results
2.1. FaSnRK1α Regulates Root Activity and Respiratory Rate under Waterlogging
2.2. FaSnRK1α Regulates Anaerobic Respiration Metabolism under Waterlogging
2.3. FaSnRK1α Regulates ROS Accumulation and Antioxidant Enzyme Activities under Waterlogging
2.4. Transcriptomic Analysis of Roots of WT and FaSnRK1α-RNAi Plants
2.5. FaSnRK1α Regulates the Expression of Anoxia-Related Genes and ERFVIIs
3. Discussion
4. Summary
5. Materials and Methods
5.1. Vector Construction and Agrobacterium-Mediated Infiltration
5.2. Plant Materials and Treatment
5.3. Determination of Root Activity and Root Respiration Rate
5.4. Determination of Respiration-Related Enzyme Activities
5.5. ROS Accumulation and Antioxidant Capacity
5.6. RNA Isolation and Library Preparation
5.7. RNA Sequencing and Differentially Expressed Gene (DEG) Analysis
5.8. RT-qPCR
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Luo, J.; Yu, W.; Xiao, Y.; Zhang, Y.; Peng, F. Strawberry FaSnRK1α Regulates Anaerobic Respiratory Metabolism under Waterlogging. Int. J. Mol. Sci. 2022, 23, 4914. https://doi.org/10.3390/ijms23094914
Luo J, Yu W, Xiao Y, Zhang Y, Peng F. Strawberry FaSnRK1α Regulates Anaerobic Respiratory Metabolism under Waterlogging. International Journal of Molecular Sciences. 2022; 23(9):4914. https://doi.org/10.3390/ijms23094914
Chicago/Turabian StyleLuo, Jingjing, Wenying Yu, Yuansong Xiao, Yafei Zhang, and Futian Peng. 2022. "Strawberry FaSnRK1α Regulates Anaerobic Respiratory Metabolism under Waterlogging" International Journal of Molecular Sciences 23, no. 9: 4914. https://doi.org/10.3390/ijms23094914