Triggering of Polymer-Degrading Enzymes from Layered Double Hydroxides for Recycling Strategies
Abstract
:1. Introduction
2. Experimental
2.1. Materials
2.2. Film Weight Loss
2.3. Immobilization of Cutinase on LDH
2.4. Triggering of Enzyme from LDH
2.5. Characterization
3. Results and Discussion
3.1. Effect of Medium on the Release of Enzyme from the LDH Structure
3.2. Effect of Sodium Phosphate Buffer Concentration and pH on the Triggering of Enzyme from the LDH Structure
4. Conclusions
5. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Triggering Solution | Concentration (M) | pH |
---|---|---|
Sodium phosphate buffer | 0.10 | 8.0 |
Citrate phosphate buffer | 0.10 | 8.0 |
Sodium sulfate (Na2SO4) | 0.08 | 7.5 |
Potassium carbonate (K2CO3) | 0.10 | 11.5 |
Sodium chloride (NaCl) | 1.00 | 7.4 |
Water (H2O) | - | 6.5 |
Triggering Solution | Degradation Rate (mgPBSA/h/cm2) | Activity (U/mL) | Relative Activity (%) |
---|---|---|---|
Sodium phosphate buffer | 6.4 | 33,975 ± 531 | 100 ± 3 |
Citrate phosphate buffer | 5.4 | 33,891 ± 1237 | 100 ± 7 |
Sodium sulfate | 0.7 | 19,546 ± 1096 | 58 ± 6 |
Potassium carbonate | 2.8 | n.a | n.a |
Sodium chloride | 0.5 | 4247 ± 163 | 12 ± 1 |
Code | T20D (°C) 1 | Residue (%) 1 | Step | |||
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | |||
Cutinase | 153 | 3 | 28% 50–190 °C | 22% 190–280 °C | 18% 280–450 °C | 29% 450–850 °C |
LDH/CO3 | 283 | 56 | 15.9% 50–220 °C | 12.6% 220–370 °C | 10.5% 370–550 °C | 4.7% 550–850 °C |
LDH/Cut-2/1 | 322 | 27 | 6% 50–190 °C | 42% 190–520 °C | 23% 520–640 °C | 2% 640–850 °C |
Triggering Solution | Protein Recovery (%) | Activity Recovery (%) | Retention Activity of Released Proteins (%) |
---|---|---|---|
Sodium phosphate buffer | 59 ± 1 | 59 ± 2 | 100 |
Citrate phosphate buffer | 69 ± 1 | 70 ± 1 | 100 |
Sodium sulfate | 57 ± 3 | 59 ± 0 | 100 |
Potassium carbonate | 69 ± 1 | 69 ± 0 | 100 |
Sodium chloride | 4 ± 0 | 2 ± 0 | 50 |
Water | 10 ± 0 | 10 ± 1 | 100 |
Triggering Solution | Protein Recovery (%) | Activity Recovery (%) | Retention Activity of Released Proteins (%) |
---|---|---|---|
Sodium phosphate buffer | 63 ± 1 | 59 ± 5 | 94 |
Citrate phosphate buffer | 63 ± 1 | 57 ± 3 | 90 |
Potassium carbonate | 60 ± 4 | 56 ± 3 | 92 |
Concentration (M) | pH | Protein Release (%) | Activity Release (%) | Retention Activity of Released Proteins (%) |
---|---|---|---|---|
0.1 | 8 | 55 ± 4 | 49 ± 3 | 89 |
0.3 | 8 | 57 ± 2 | 47 ± 4 | 83 |
0.5 | 8 | 59 ± 1 | 50 ± 3 | 85 |
Concentration (M) | pH | Protein Release (%) | Activity Release (%) | Retention Activity of Released Proteins (%) |
---|---|---|---|---|
0.1 | 8 | 55 ± 4 | 49 ± 3 | 89 |
0.1 | 6 | 50 ± 3 | 43 ± 3 | 86 |
0.1 | 4 | 52 ± 4 | 47 ± 3 | 90 |
Solution | Degradation Rate (mgPBSA/h/cm2) |
---|---|
Sodium phosphate buffer 0.3 M pH 8 | 6.0 |
Sodium phosphate buffer 0.5 M pH 8 | 6.2 |
Sodium phosphate buffer 0.1 M pH 8 | 6.4 |
Sodium phosphate buffer 0.1 M pH 4 | 5.6 |
Sodium phosphate buffer 0.1 M pH 6 | 6.2 |
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Romano, A.; Rosato, A.; Bianchi, S.; Zanaroli, G.; Celli, A.; Totaro, G.; Sisti, L. Triggering of Polymer-Degrading Enzymes from Layered Double Hydroxides for Recycling Strategies. Int. J. Mol. Sci. 2023, 24, 831. https://doi.org/10.3390/ijms24010831
Romano A, Rosato A, Bianchi S, Zanaroli G, Celli A, Totaro G, Sisti L. Triggering of Polymer-Degrading Enzymes from Layered Double Hydroxides for Recycling Strategies. International Journal of Molecular Sciences. 2023; 24(1):831. https://doi.org/10.3390/ijms24010831
Chicago/Turabian StyleRomano, Angela, Antonella Rosato, Stefano Bianchi, Giulio Zanaroli, Annamaria Celli, Grazia Totaro, and Laura Sisti. 2023. "Triggering of Polymer-Degrading Enzymes from Layered Double Hydroxides for Recycling Strategies" International Journal of Molecular Sciences 24, no. 1: 831. https://doi.org/10.3390/ijms24010831