Simple Coumarins from Peucedanum luxurians Fruits: Evaluation of Anxiolytic Activity and Influence on Gene Expression Related to Anxiety in Zebrafish Model
Abstract
:1. Introduction
2. Results
2.1. The Effects of Isolated Coumarins on Thigmotaxis Behaviors of the Zebrafish Larvae during Light–Dark Changes
2.1.1. The Effects of Officinalin on Locomotor Activity and Thigmotaxis Behaviors of the Zebrafish Larvae during Light–Dark Changes
2.1.2. The Effects of Stenocarpin Isobutyrate on Locomotor Activity and Thigmotaxis Behaviors of the Zebrafish Larvae during Light–Dark Changes
2.1.3. The Effects of Officinalin Isobutyrate on Locomotor Activity and Thigmotaxis Behaviors of the Zebrafish Larvae during Light–Dark Changes
2.2. Influence of Isolated Coumarins on the Expression of Genes Associated with the Etiology and Extinction of Anxiety
3. Discussion
4. Materials and Methods
4.1. Drugs
4.2. Zebrafish Model for Evaluation of Anxiety-Like Behaviors
4.2.1. Animal Husbandry
4.2.2. Determination of the Maximum Tolerated Concentration
4.2.3. Anxiolytic Activity Assay
4.3. RNA Isolation and Quantitative PCR
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Forward 5′-3′ | Reverse 5′-3′ | Accession No/Source |
---|---|---|---|
c-fos | GTGCAGCACGGCTTCACCGA | TTGAGCTGCGCCGTTGGAGG | NM_205569.1 |
th1 | GACGGAAGATGATCGGAGACA | CCGCCATGTTCCGATTTCT | NM_131149.1/ |
gal | GACCAACTGATACTCAGGATGCA | ATCCCGAGTGTTTCTGTCAGAA | NM_001346239.1 |
gabrapa | AGGATCCACTTGAGGGCTGA | CTTCTTCGTGATGCTCCTGGT | NM_001013260.1/ |
gabarapb | TGTAAACAACGTCATTCCCCCT | ATCTTCTTCGTGATGTTCCTGGT | NM_001386387.1 |
penka | CTTTGAGCGCCTGTCTCGTG | TTCTAATGTGCAGGCCAGTGTG | NM_200083.2 |
penkb | TGGTGGCAGGAGTCTAAACG | TTGGACATCGCGGACATCAT | NM_182883.1 |
htr1aa | CAGAGCAGAGCAGCACAAG | TGGTCTGAGAGTTCTGGTCTAATC | NM_001123321.1 |
htr1b | GTGTCGGTGCTCGTGATG | CAGCCAGATGTCGCAGATG | NM_001128709.1 |
htr2b | GCTGCTCATTCTTCTGGTCAT | GTTAGTGGCGTTCTGGAGTT | NM_001044743.1 |
bdnf | GGCGAAGAGCGGACGAATATC | AAGGAGACCATTCAGCAGGACAG | NM_131595.2 |
Ef1α | CTGGAGGCCAGCTCAAACAT | ATCAAGAAGAGTAGTACCGCTAGCATTAC | NM_131263.1 |
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Widelski, J.; Kasica, N.; Maciąg, M.; Luca, S.V.; Budzyńska, B.; Fondai, D.; Podlasz, P.; Skalicka-Woźniak, K. Simple Coumarins from Peucedanum luxurians Fruits: Evaluation of Anxiolytic Activity and Influence on Gene Expression Related to Anxiety in Zebrafish Model. Int. J. Mol. Sci. 2023, 24, 8693. https://doi.org/10.3390/ijms24108693
Widelski J, Kasica N, Maciąg M, Luca SV, Budzyńska B, Fondai D, Podlasz P, Skalicka-Woźniak K. Simple Coumarins from Peucedanum luxurians Fruits: Evaluation of Anxiolytic Activity and Influence on Gene Expression Related to Anxiety in Zebrafish Model. International Journal of Molecular Sciences. 2023; 24(10):8693. https://doi.org/10.3390/ijms24108693
Chicago/Turabian StyleWidelski, Jarosław, Natalia Kasica, Monika Maciąg, Simon Vlad Luca, Barbara Budzyńska, Dafina Fondai, Piotr Podlasz, and Krystyna Skalicka-Woźniak. 2023. "Simple Coumarins from Peucedanum luxurians Fruits: Evaluation of Anxiolytic Activity and Influence on Gene Expression Related to Anxiety in Zebrafish Model" International Journal of Molecular Sciences 24, no. 10: 8693. https://doi.org/10.3390/ijms24108693