Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from Nymphaea hybrid
Abstract
:1. Introduction
2. Results and Discussion
2.1. Single Factors Experiment Analysis
2.2. Optimization of Extraction Parameters
2.2.1. Optimization of NHE Yield by RSM
2.2.2. Analysis of Response Surfaces
2.2.3. Verification
2.3. Component Analyses
2.4. Fourier Transform Infrared Spectroscopy Analysis
2.5. Thermal Property Analysis
2.6. DPPH Radical Scavenging Activity
2.7. Hyaluronidase Activity Inhibition
2.8. Effect of NHE on ROS Level of HaCaT Cells
2.9. Effect of NHE on Melanin Production of B16 Cells
2.10. Re-Epithelialization of NHE by Scratch Assay
3. Materials and Methods
3.1. Materials and Reagent
3.2. Preparation of NHE and Single Factors Experiment
3.3. Response Surface Experiment
3.4. Determination of Component
3.5. Characterization of NHE by HPLC and FT–IR
3.6. Thermal (TG–DTG) Analysis of NHE
3.7. Antioxidant and Anti-Inflammatory Activity of NHE
3.8. Cells Culture and Cytotoxicity Assay of NHE
3.9. Measurement of ROS Production
3.10. Measurement of Melanin Content
3.11. Wound Healing Scratch Migration Assay
3.12. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Run | A | B | C | Yield (%) |
---|---|---|---|---|
1 | 40 | 20 | 50 | 6.47 ± 0.03 |
2 | 30 | 30 | 50 | 8.11 ± 0.08 |
3 | 20 | 40 | 50 | 6.55 ± 0.11 |
4 | 40 | 40 | 50 | 7.31 ± 0.09 |
5 | 30 | 20 | 55 | 6.79 ± 0.09 |
6 | 40 | 30 | 45 | 7.22 ± 0.13 |
7 | 30 | 20 | 45 | 7.14 ± 0.18 |
8 | 30 | 30 | 50 | 7.98 ± 0.08 |
9 | 30 | 30 | 50 | 8.16 ± 0.09 |
10 | 30 | 30 | 50 | 8.20 ± 0.21 |
11 | 30 | 40 | 45 | 7.75 ± 0.15 |
12 | 30 | 40 | 55 | 6.63 ± 0.09 |
13 | 30 | 30 | 50 | 7.88 ± 0.12 |
14 | 40 | 30 | 55 | 6.74 ± 0.13 |
15 | 20 | 30 | 45 | 7.35 ± 0.05 |
16 | 20 | 20 | 50 | 6.19 ± 0.14 |
17 | 20 | 30 | 55 | 6.04 ± 0.09 |
Variables | Sum of Squares | df | Mean Square | F-Value | p-Value Prob. > F |
---|---|---|---|---|---|
Model | 8.01 | 9 | 0.89 | 34.47 | <0.0001 *** |
A | 0.33 | 1 | 0.33 | 12.73 | 0.0091 ** |
B | 0.34 | 1 | 0.34 | 13.16 | 0.0084 ** |
C | 1.33 | 1 | 1.33 | 51.54 | 0.0002 *** |
AB | 0.058 | 1 | 0.06 | 2.26 | 0.0176 * |
AC | 0.17 | 1 | 0.17 | 6.70 | 0.0360 * |
BC | 0.15 | 1 | 0.15 | 5.64 | 0.0492 * |
A2 | 2.95 | 1 | 2.95 | 114.36 | <0.0001 *** |
B2 | 1.51 | 1 | 1.51 | 58.55 | 0.0001 *** |
C2 | 0.64 | 1 | 0.64 | 24.78 | 0.0016 ** |
Residual | 0.18 | 7 | 0.035 | 2.00 | |
Lack of fit | 0.11 | 3 | 0.036 | 34.47 | 0.2558 |
Pure error | 0.072 | 4 | 0.034 |
Physicochemical Property | Proportion (w/w%) |
---|---|
Neutral sugar | 41.31 ± 0.24 |
Uronic acid | 41.68 ± 0.42 |
Protein | 0.09 ± 0.01 |
Polyphenol | 2.20 ± 0.11 |
Yield | 8.22 ± 0.16 |
Monosaccharide Composition | Peak Area (%) |
---|---|
Man | 4.555 |
Rib | 0.139 |
Rha | 5.655 |
GlcUA | 3.946 |
GalUA | 47.311 |
Glc | 6.811 |
Gal | 18.612 |
Xyl | 6.323 |
Ara | 6.382 |
Fuc | 0.265 |
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Liu, H.-M.; Tang, W.; Lei, S.-N.; Zhang, Y.; Cheng, M.-Y.; Liu, Q.-L.; Wang, W. Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from Nymphaea hybrid. Int. J. Mol. Sci. 2023, 24, 8974. https://doi.org/10.3390/ijms24108974
Liu H-M, Tang W, Lei S-N, Zhang Y, Cheng M-Y, Liu Q-L, Wang W. Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from Nymphaea hybrid. International Journal of Molecular Sciences. 2023; 24(10):8974. https://doi.org/10.3390/ijms24108974
Chicago/Turabian StyleLiu, Hui-Min, Wei Tang, Sheng-Nan Lei, Yun Zhang, Ming-Yan Cheng, Qing-Lei Liu, and Wei Wang. 2023. "Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from Nymphaea hybrid" International Journal of Molecular Sciences 24, no. 10: 8974. https://doi.org/10.3390/ijms24108974