Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP)
Abstract
:1. Introduction
2. Results
2.1. Dose-Response Dependence for Cell Membrane Permeabilization by 7 kV/cm, 300-ns Pulses
2.2. Validation of CRISPR-Cas9 Genome Editing
2.3. Effect of Gene KOs on YP Uptake by Electropermeabilized Cells
2.4. The Correlation of Gene Expression with Cell Survival
3. Discussion
4. Materials and Methods
4.1. Reagents and Materials
4.2. Cell Culture and Gene Knock-Out Procedures
4.3. Genomic Cleavage Detection
4.4. Electroporation and Detection of Cell Membrane Permeabilization
4.5. Image Analysis, Quantification of Membrane Permeabilization, and Hit Selection
4.6. Gene Expression Analysis in Cell Lines
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Silkuniene, G.; Mangalanathan, U.M.; Rossi, A.; Mollica, P.A.; Pakhomov, A.G.; Pakhomova, O. Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP). Int. J. Mol. Sci. 2023, 24, 9191. https://doi.org/10.3390/ijms24119191
Silkuniene G, Mangalanathan UM, Rossi A, Mollica PA, Pakhomov AG, Pakhomova O. Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP). International Journal of Molecular Sciences. 2023; 24(11):9191. https://doi.org/10.3390/ijms24119191
Chicago/Turabian StyleSilkuniene, Giedre, Uma M. Mangalanathan, Alessandra Rossi, Peter A. Mollica, Andrei G. Pakhomov, and Olga Pakhomova. 2023. "Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP)" International Journal of Molecular Sciences 24, no. 11: 9191. https://doi.org/10.3390/ijms24119191