Transcription Factor MdbHLH093 Enhances Powdery Mildew Resistance by Promoting Salicylic Acid Signaling and Hydrogen Peroxide Accumulation
Abstract
:1. Introduction
2. Results
2.1. Sequence Analysis and Evolutionary Tree Construction of MdbHLH093
2.2. Expression Pattern Analysis of MdbHLH093
2.3. Heterologous Expression of MdbHLH093 in A. thaliana Enhances Resistance to Powdery Mildew
2.4. MdbHLH093-Overexpressing Plants Increase the Expression of Genes Related to the SA Signaling Pathway
2.5. Transient MdbHLH093 Overexpression Enhances Apple Leaf Resistance to Powdery Mildew
2.6. Transient Silencing of MdbHLH093 Reduces Apple Resistance to Powdery Mildew
2.7. MdbHLH093 Interacts with MdMYB116
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Pathogen Inoculation
4.3. Bioinformatics Analysis
4.4. Subcellular Localization Analysis
4.5. Arabidopsis Transformation and Disease Analysis
4.6. Instantaneous Transformation of Apple Leaves Mediated by Agrobacterium tumefaciens and Inoculation with Powdery Mildew
4.7. Yeast Two-Hybrid Assay
4.8. Bimolecular Fluorescence Complementary Analysis
4.9. Split Luciferase Assay
4.10. Gene Expression Analysis by RT-qPCR
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ma, H.; Zou, F.; Li, D.; Wan, Y.; Zhang, Y.; Zhao, Z.; Wang, X.; Gao, H. Transcription Factor MdbHLH093 Enhances Powdery Mildew Resistance by Promoting Salicylic Acid Signaling and Hydrogen Peroxide Accumulation. Int. J. Mol. Sci. 2023, 24, 9390. https://doi.org/10.3390/ijms24119390
Ma H, Zou F, Li D, Wan Y, Zhang Y, Zhao Z, Wang X, Gao H. Transcription Factor MdbHLH093 Enhances Powdery Mildew Resistance by Promoting Salicylic Acid Signaling and Hydrogen Peroxide Accumulation. International Journal of Molecular Sciences. 2023; 24(11):9390. https://doi.org/10.3390/ijms24119390
Chicago/Turabian StyleMa, Hai, Fuyan Zou, Dongmei Li, Ye Wan, Yiping Zhang, Zhengyang Zhao, Xiping Wang, and Hua Gao. 2023. "Transcription Factor MdbHLH093 Enhances Powdery Mildew Resistance by Promoting Salicylic Acid Signaling and Hydrogen Peroxide Accumulation" International Journal of Molecular Sciences 24, no. 11: 9390. https://doi.org/10.3390/ijms24119390