Modulation of Dietary Choline Uptake in a Mouse Model of Acid Sphingomyelinase Deficiency
Abstract
:1. Introduction
2. Results
2.1. Choline-Free Diet Shows No Overt Toxicity and Does Not Affect Liver to Body Weight Ratio in ASMko Mice
2.2. Choline-Free Diet Alters Lipid Composition in the Liver, Mainly by Changing Levels of Sphingolipids and Glycerophospholipids with 34:1 Fatty Acids
2.3. Choline-Free Diet Reduces Inflammation in the Liver of ASMko Mice
2.4. Choline Deficiency Had Minor Effects on Brain Lipid Composition
2.5. Choline-Free Diet Reduces Neuroinflammation but Not Cell Death in the Brain of ASMko Mice
3. Discussion
4. Materials and Methods
4.1. Antibodies
4.2. Mice
4.3. Choline-Free Diet Treatment
4.4. Behavioural Tests
4.5. Liver Weight Assessment
4.6. Lipidomic Profiling
4.7. Immunohistochemistry
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gaudioso, Á.; Moreno-Huguet, P.; Casas, J.; Schuchman, E.H.; Ledesma, M.D. Modulation of Dietary Choline Uptake in a Mouse Model of Acid Sphingomyelinase Deficiency. Int. J. Mol. Sci. 2023, 24, 9756. https://doi.org/10.3390/ijms24119756
Gaudioso Á, Moreno-Huguet P, Casas J, Schuchman EH, Ledesma MD. Modulation of Dietary Choline Uptake in a Mouse Model of Acid Sphingomyelinase Deficiency. International Journal of Molecular Sciences. 2023; 24(11):9756. https://doi.org/10.3390/ijms24119756
Chicago/Turabian StyleGaudioso, Ángel, Pilar Moreno-Huguet, Josefina Casas, Edward H. Schuchman, and María Dolores Ledesma. 2023. "Modulation of Dietary Choline Uptake in a Mouse Model of Acid Sphingomyelinase Deficiency" International Journal of Molecular Sciences 24, no. 11: 9756. https://doi.org/10.3390/ijms24119756