Thermochemical Study of the Interaction of Cytosine and Uracil with Peptides in a Buffered Saline: Complex Formation with beta-Endorphin 30-31 (Human), L-Glutathion (Reduced) and α-L-Alanyl-L-Tyrosine
Abstract
:1. Introduction
- (a)
- To obtain thermochemical characteristics of the interaction of uracil and cytosine with a number of peptides of various structures by calorimetry of dissolution;
- (b)
- To calculate the values of the complexation constant, and the change in Gibbs energy, enthalpy, and entropy from calorimetric data;
- (c)
- To study the effect of the charge and the number of donors and acceptors of the H-bond in the structure of peptide on the patterns of binding to nucleic bases.
2. Results and Discussion
2.1. Distribution of Ionic Forms of Peptides
2.2. Enthalpies of Dissolution of Peptides
2.3. Thermodynamic Parameters of Complexation of Peptides with Nucleic Bases
3. Materials and Methods
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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mNB | ΔsolHm | mNB | ΔsolHm |
---|---|---|---|
GlyGlu + uracil | GlyGlu + cytosine | ||
0 | 11.43 | 0.0030 | 11.12 |
0.0015 | 11.42 | 0.0091 | 10.53 |
0.0033 | 11.39 | 0.0153 | 10.03 |
0.0062 | 11.37 | 0.0185 | 9.66 |
0.0090 | 11.35 | 0.0242 | 9.28 |
0.0151 | 11.30 | 0.0303 | 8.73 |
0.0212 | 11.25 | 0.0378 | 8.24 |
0.0272 | 11.20 | 0.0480 | 7.68 |
0.0292 | 11.21 | 0.0599 | 7.11 |
γGluCysGly + uracil | γGluCysGly + cytosine | ||
0 | 20.36 | 0.0030 | 20.05 |
0.0030 | 19.64 | 0.0090 | 19.53 |
0.0055 | 19.00 | 0.0150 | 19.12 |
0.0090 | 18.48 | 0.0210 | 18.78 |
0.0116 | 18.03 | 0.0240 | 18.64 |
0.0151 | 17.67 | 0.0300 | 18.38 |
0.0212 | 17.24 | 0.0344 | 18.18 |
0.0272 | 17.21 | 0.0420 | 17.98 |
0.0303 | 17.28 | ||
AlaTyr + uracil | AlaTyr + cytosine | ||
0 | 10.89 | 0.0050 | 11.59 |
0.0050 | 10.33 | 0.0080 | 12.04 |
0.0078 | 10.06 | 0.0115 | 12.59 |
0.0115 | 9.74 | 0.0155 | 12.97 |
0.0155 | 9.44 | 0.0175 | 13.21 |
0.0180 | 9.27 | 0.0201 | 13.47 |
0.0201 | 9.13 | 0.0250 | 13.84 |
0.0250 | 8.86 | 0.0271 | 13.94 |
0.0300 | 8.61 | ||
0.0320 | 8.53 | ||
AlaAla + uracil | |||
0 | −7.46 | ||
0.0050 | −7.44 | ||
0.0078 | −7.41 | ||
0.0115 | −7.36 | ||
0.0155 | −7.32 | ||
0.0201 | −7.27 | ||
0.0250 | −7.25 | ||
0.0300 | −7.24 | ||
0.0320 | −7.23 |
lgKr * | ΔrG | ΔrH | T ΔrS | |
---|---|---|---|---|
kJ·mol−1 | ||||
Ur + GlyGlu | 1.16 ± 0.001 | −6.64 ± 0.01 | −0.7 ± 0.1 | 5.9 ± 0.1 |
Ur + γ-GluCysGly | 2.01 ± 0.04 | −11.4 ± 0.2 | −4.4 ± 0.1 | 7.0 ± 0.3 |
Ur + AlaTyr | 1.45 ± 0.001 | −8.3 ± 0.01 | −5.30 ± 0.05 | 3.0 ± 0.06 |
Ur + AlaAla | 1.03 ± 0.05 | −5.9 ± 0.3 | 1.0 ± 0.04 | 6.9 ± 0.3 |
Cyt + GlyGlu | 0.95 ± 0.001 | −5.40 ± 0.01 | −12.7 ± 0.08 | −7.3 ± 0.1 |
Cyt + γ-GluCysGly | 1.39 ± 0.002 | −7.89 ± 0.01 | −4.79 ± 0.07 | 3.1 ± 0.1 |
Cyt + AlaTyr | 1.29 ± 0.002 | −7.35 ± 0.01 | 9.8 ± 0.1 | 17.15 ± 0.1 |
Chemical a | M b | CAS No. c | Origin | Purity d |
---|---|---|---|---|
Uracil | 112.09 | 66-22-8 | Termo Ficher Scientific | 0.996 |
Cytosine | 111.10 | 70-30-7 | Appolo Scientific | 0.996 |
Glycyl-L-glutamic acid (beta-endorphin 30-31 human) | 204.18 | 7412-78-4 | Bachem | >0.99 |
L-γ-Glutamyl-L-cysteinyl-glycine (glutathion reduced) | 307.32 | 108457-42-7 | Tokyo Chemical Industry | >0.99 |
L-α-Alanyl-L-tyrosine | 252.27 | 3061-88-9 | Abcr GmbH | >0.98 |
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Barannikov, V.P.; Smirnov, V.I.; Mezhevoi, I.N.; Koltyshev, D.R. Thermochemical Study of the Interaction of Cytosine and Uracil with Peptides in a Buffered Saline: Complex Formation with beta-Endorphin 30-31 (Human), L-Glutathion (Reduced) and α-L-Alanyl-L-Tyrosine. Int. J. Mol. Sci. 2023, 24, 9764. https://doi.org/10.3390/ijms24119764
Barannikov VP, Smirnov VI, Mezhevoi IN, Koltyshev DR. Thermochemical Study of the Interaction of Cytosine and Uracil with Peptides in a Buffered Saline: Complex Formation with beta-Endorphin 30-31 (Human), L-Glutathion (Reduced) and α-L-Alanyl-L-Tyrosine. International Journal of Molecular Sciences. 2023; 24(11):9764. https://doi.org/10.3390/ijms24119764
Chicago/Turabian StyleBarannikov, Vladimir P., Valeriy I. Smirnov, Igor N. Mezhevoi, and Damir R. Koltyshev. 2023. "Thermochemical Study of the Interaction of Cytosine and Uracil with Peptides in a Buffered Saline: Complex Formation with beta-Endorphin 30-31 (Human), L-Glutathion (Reduced) and α-L-Alanyl-L-Tyrosine" International Journal of Molecular Sciences 24, no. 11: 9764. https://doi.org/10.3390/ijms24119764