STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression
Abstract
:1. Introduction
2. Results
2.1. ID1 Expression Level Is Up-Regulated in CRC
2.2. ID1 Promotes CRC Cell Proliferation and Colony Formation
2.3. Identification of ID1-Associated Enhancers in CRC
2.4. STAT3 Promotes Expression of ID1 and Enhancer Activity in CRC Cells
2.5. E1 Enhancer Knockout Reduces ID1 Expression and Inhibits Proliferation of CRC Cells
3. Discussion
4. Materials and Methods
4.1. Patients and Tissues
4.2. Immunohistochemistry (IHC)
4.3. Cell Culture and Treatment
4.4. RNA Extraction, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Quantitative PCR (qPCR)
4.5. Western Blot Assay
4.6. Microarray, ChIP-seq and GRO-seq Data Analyses
4.7. Chromatin Immuno-Precipitation (ChIP)
4.8. CRISPR/Cas9-Mediated ID1 and Enhancer E1 Deletion
4.9. Cell Proliferation Assay
4.10. Colony-Forming Assay
4.11. Transwell Assay
4.12. Dual-Luciferase Reporter Assay
4.13. Chromosome Conformation Capture (3C) Assay
4.14. Animal Studies
4.15. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Lin, Z.; Liu, Y.; Xu, T.; Su, T.; Yang, Y.; Liang, R.; Gu, S.; Li, J.; Song, X.; Liang, B.; et al. STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression. Int. J. Mol. Sci. 2023, 24, 10041. https://doi.org/10.3390/ijms241210041
Lin Z, Liu Y, Xu T, Su T, Yang Y, Liang R, Gu S, Li J, Song X, Liang B, et al. STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression. International Journal of Molecular Sciences. 2023; 24(12):10041. https://doi.org/10.3390/ijms241210041
Chicago/Turabian StyleLin, Zhike, Ying Liu, Tian Xu, Ting Su, Yingying Yang, Runhua Liang, Songgang Gu, Jie Li, Xuhong Song, Bin Liang, and et al. 2023. "STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression" International Journal of Molecular Sciences 24, no. 12: 10041. https://doi.org/10.3390/ijms241210041