Hif1α/Dhrs3a Pathway Participates in Lipid Droplet Accumulation via Retinol and Ppar-γ in Fish Hepatocytes
Abstract
:1. Introduction
2. Results
2.1. LD Accumulation Is Accompanied by Increased Dhrs3 Expression
2.2. Dhrs3a Knockdown Delays LD Accumulation in Cells in a Lipid-Rich Medium
2.3. Exogenous Retinyl Acetate Maintains LD Accumulation
2.4. Exogenous Retinyl Acetate Alters the Lipidome of Zebrafish
2.5. Hif1α Regulates dhrs3a and LD Accumulation
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. RNA Interference and Treatment with an Inhibitor
4.3. LD Staining
4.4. TG and Retinol Content Assay
4.5. Immunofluorescence Staining
4.6. Transcriptome Analysis
4.7. qRT-PCR
4.8. Lipidomic Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tian, J.; Du, Y.; Wang, B.; Ji, M.; Li, H.; Xia, Y.; Zhang, K.; Li, Z.; Xie, W.; Gong, W.; et al. Hif1α/Dhrs3a Pathway Participates in Lipid Droplet Accumulation via Retinol and Ppar-γ in Fish Hepatocytes. Int. J. Mol. Sci. 2023, 24, 10236. https://doi.org/10.3390/ijms241210236
Tian J, Du Y, Wang B, Ji M, Li H, Xia Y, Zhang K, Li Z, Xie W, Gong W, et al. Hif1α/Dhrs3a Pathway Participates in Lipid Droplet Accumulation via Retinol and Ppar-γ in Fish Hepatocytes. International Journal of Molecular Sciences. 2023; 24(12):10236. https://doi.org/10.3390/ijms241210236
Chicago/Turabian StyleTian, Jingjing, Yihui Du, Binbin Wang, Mengmeng Ji, Hongyan Li, Yun Xia, Kai Zhang, Zhifei Li, Wenping Xie, Wangbao Gong, and et al. 2023. "Hif1α/Dhrs3a Pathway Participates in Lipid Droplet Accumulation via Retinol and Ppar-γ in Fish Hepatocytes" International Journal of Molecular Sciences 24, no. 12: 10236. https://doi.org/10.3390/ijms241210236