Epidermal Loss of RORα Enhances Skin Inflammation in a MC903-Induced Mouse Model of Atopic Dermatitis
Abstract
:1. Introduction
2. Results
2.1. Epidermal Rora Expression in Mouse Skin in Response to MC903
2.2. Epidermal Rora Ablation Exaggerates MC903-Induced Ear Thickening
2.3. Epidermal Rora Deletion Aggravates MC903-Induced AD-like Symptoms
2.4. Epidermal Loss of RORα Accelerates MC903-Induced Dermal Activation of Dendritic Cells and CD4+ T Cells
2.5. Epidermal Rora Deletion Increases Tslp Gene Transcription
2.6. Epidermal Loss of RORα Deregulates Keratinocyte Differentiation and Accelerates Skin Barrier Disruption in Response to MC903
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Genotyping
4.3. MC903-Induced AD-like Model
4.4. X-Gal Staining
4.5. Histology and Immunofluorescence
4.6. Quantitative Real-Time (RT)-PCR
4.7. ELISA
4.8. Transepidermal Water Loss Measurements
4.9. Western Blot Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hua, X.; Blosch, C.D.; Dorsey, H.; Ficaro, M.K.; Wallace, N.L.; Hsung, R.P.; Dai, J. Epidermal Loss of RORα Enhances Skin Inflammation in a MC903-Induced Mouse Model of Atopic Dermatitis. Int. J. Mol. Sci. 2023, 24, 10241. https://doi.org/10.3390/ijms241210241
Hua X, Blosch CD, Dorsey H, Ficaro MK, Wallace NL, Hsung RP, Dai J. Epidermal Loss of RORα Enhances Skin Inflammation in a MC903-Induced Mouse Model of Atopic Dermatitis. International Journal of Molecular Sciences. 2023; 24(12):10241. https://doi.org/10.3390/ijms241210241
Chicago/Turabian StyleHua, Xiangmei, Conrad Dean Blosch, Hannah Dorsey, Maria K. Ficaro, Nicole L. Wallace, Richard P. Hsung, and Jun Dai. 2023. "Epidermal Loss of RORα Enhances Skin Inflammation in a MC903-Induced Mouse Model of Atopic Dermatitis" International Journal of Molecular Sciences 24, no. 12: 10241. https://doi.org/10.3390/ijms241210241