Small RNA Sequencing Analysis of STZ-Injured Pancreas Reveals Novel MicroRNA and Transfer RNA-Derived RNA with Biomarker Potential for Diabetes Mellitus
Abstract
:1. Introduction
2. Results
2.1. Fasting Blood Glucose Level
2.2. The H&E Staining of the Pancreas in DM Model and Normal Rats
2.3. Profiling and Characterization of tsRNAs in Normal and Model Groups
2.4. Differentially Expressed miRNAs and tsRNAs between Normal and Model Groups
2.5. qRT-PCR Validation of Differentially Expressed miRNAs and tsRNAs
2.6. Identification of miRNA and tsRNA Target Genes
2.7. Enrichment Analysis for the Target Genes of Differentially Expressed miRNAs and tsRNAs
3. Discussion
4. Materials and Methods
4.1. Animal Experiment and Tissue Extraction
4.2. Histological Examination of Pancreatic Tissues
4.3. RNA Isolation and Small RNA-Seq Anaysis
4.4. Data Collection and Analysis
4.5. Validation of Small RNA-Seq Analysis Using qRT-PCR
4.6. Differential tsRNA and miRNA Target Gene Prediction and Functional Prediction
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | FBG Level (mmol/L) | p |
---|---|---|
Normal | 4.47 ± 0.65 | p < 0.0001 |
Model | 15.61 ± 3.29 |
miRNA | Length | Regulation | Fold_Change | p_Value |
---|---|---|---|---|
rno-miR-19a-5p | 20 | up | 18.412984 | 0.0384746 |
rno-miR-138-2-3p | 21 | up | 8.7834281 | 0.024325 |
rno-miR-34a-3p | 22 | up | 3.8157119 | 0.0380204 |
rno-miR-542-5p | 22 | up | 3.0840682 | 0.0222089 |
rno-miR-34a-5p | 22 | up | 2.069457 | 0.0016151 |
rno-miR-3594-5p | 21 | down | 0.063501 | 0.0453837 |
rno-miR-448-3p | 21 | down | 0.2210338 | 0.0390464 |
rno-miR-369-5p | 22 | down | 0.3206308 | 0.0330345 |
rno-miR-137-3p | 23 | down | 0.4342942 | 0.0228723 |
rno-miR-384-5p | 23 | down | 0.4461936 | 0.013194 |
tsRNA | Type | Regulation | Length | Fold_Change | p_Value |
---|---|---|---|---|---|
tRF-Arg-ACG-007 | tRF-3a | up | 18 | 9.8496924 | 0.022108267 |
tRF-Thr-AGT-013 | tRF-3a | up | 17 | 8.217405 | 0.0091561 |
tiRNA-His-GTG-002 | tiRNA-3 | up | 40 | 7.3252156 | 1.00809 × 10−07 |
tRF-Gly-GCC-018 | tRF-3b | up | 22 | 4.042935 | 0.004316473 |
tRF-Asn-GTT-031 | tRF-2 | up | 14 | 3.7170533 | 0.032505749 |
tRF-Ser-GCT-008 | tRF-5c | down | 28 | 0.0451683 | 0.024084927 |
tRF-Trp-TCA-001 | tRF-3b | down | 21 | 0.0616459 | 0.003398912 |
tRF-Trp-TCA-002 | tRF-3b | down | 20 | 0.0650623 | 0.013008669 |
tRF-His-GTG-037 | tRF-2 | down | 14 | 0.0895452 | 0.006455851 |
tRF-Arg-TCG-016 | tRF-2 | down | 14 | 0.1075931 | 0.010376773 |
Primer Name | Sequence |
---|---|
rno-miR-34a-5p | F:5′GGGGTGGCAGTGTCTTAGC3′ R:5′GTGCGTGTCGTGGAGTCG3′ |
rno-miR-181c-3p | F:5′GGACCATCGACCGTTGAG3′ R:5′GTGCGTGTCGTGGAGTCG3′ |
rno-miR-182 | F:5′GGCTTTGGCAATGGTAGAAC3′ R:5′GTGCGTGTCGTGGAGTCG3′ |
rno-miR-384-5p | F:5′GGGGGTTGTAAACAATTCCTAG R:5′GTGCGTGTCGTGGAGTCG3′ |
tRF-His-GTG-029 | F:5′GATCGCCGTGATCGTATAGTG3′ R:5′TCTTCCGATCTACGCAGAGTACTA3′ |
tiRNA-iMet-CAT-001 | F:5′CGATCAGCAGAGTGGCGCAG3′ R:5′ATCTGGGCCCAGCACGCTT3′ |
tRF-Met-CAT-012 | F:5′TCCGACGATCAGTAAGGTCA3′ R:5′GATCTGGCCCGATAGCTTAG3′ |
tRF-Lys-TTT-024 | F:5′ACGATCGCCCGGATAGCT3′ R:5′CCGATCTTGATGCTCTACCGA3′ |
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Mo, F.; Lv, B.; Zhao, D.; Xi, Z.; Qian, Y.; Ge, D.; Yang, N.; Zhang, D.; Jiang, G.; Gao, S. Small RNA Sequencing Analysis of STZ-Injured Pancreas Reveals Novel MicroRNA and Transfer RNA-Derived RNA with Biomarker Potential for Diabetes Mellitus. Int. J. Mol. Sci. 2023, 24, 10323. https://doi.org/10.3390/ijms241210323
Mo F, Lv B, Zhao D, Xi Z, Qian Y, Ge D, Yang N, Zhang D, Jiang G, Gao S. Small RNA Sequencing Analysis of STZ-Injured Pancreas Reveals Novel MicroRNA and Transfer RNA-Derived RNA with Biomarker Potential for Diabetes Mellitus. International Journal of Molecular Sciences. 2023; 24(12):10323. https://doi.org/10.3390/ijms241210323
Chicago/Turabian StyleMo, Fangfang, Bohan Lv, Dandan Zhao, Ziye Xi, Yining Qian, Dongyu Ge, Nan Yang, Dongwei Zhang, Guangjian Jiang, and Sihua Gao. 2023. "Small RNA Sequencing Analysis of STZ-Injured Pancreas Reveals Novel MicroRNA and Transfer RNA-Derived RNA with Biomarker Potential for Diabetes Mellitus" International Journal of Molecular Sciences 24, no. 12: 10323. https://doi.org/10.3390/ijms241210323