CCN3/NOV Regulates Proliferation and Neuronal Differentiation in Mouse Hippocampal Neural Stem Cells via the Activation of the Notch/PTEN/AKT Pathway
Abstract
:1. Introduction
2. Results
2.1. CCN3 Promotes the Cell Viability of Cultured Neonatal Mouse Hippocampal NSCs
2.2. CCN3 Promotes Nsc Proliferation in Mouse Hippocampus
2.3. CCN3 Inhibits Neuronal Differentiation of Nscs in Mouse Hippocampus
2.4. CCN3 Regulates the Proliferation and Neuronal Differentiation of Cultured Mouse Hippocampal NSCs
2.5. CCN3 Regulates Proliferation and Neuronal Differentiation via Notch/Pten/Akt Pathway
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Primary Hippocampal NSCs Culture
4.3. Immunostaining
4.4. Lentiviral Transfection
4.5. Stereotaxic Surgery
4.6. Experimental Treatments
4.7. BrdU Incorporation
4.8. Cell Cycle Analysis
4.9. Cell Viability Assay
4.10. TUNEL Staining
4.11. Western Blot Analysis
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
NSCs | neural stem cells |
SGZ | subgranular zone |
CCN | cellular communication network |
CCN3 | cellular communication network protein 3 |
DG | dentate gyrus |
PI | proliferation index |
NOV | nephroblastoma overexpressed |
Tuj1 | class III beta tubulin |
DCX | Doublecortin |
BrdU | 5-Bromo-2′-deoxyuridine |
CCK-8 | Cell Counting Kit-8 |
MSCs | mesenchymal stem cells |
HSC | Hematopoietic stem cell |
GNP | granule neuron precursors |
EGF | epidermal growth factor |
bFGF | basic fibroblast growth factor |
PDL | Poly-D-lysine |
PFA | paraformaldehyde |
CNS | central nerve system |
LRP1 | low-density-lipoprotein receptor-related protein 1 |
PIP3 | Phosphatidylinositol-3,4,5- trisphosphate |
PIP2 | Phosphatidylinositol (4,5)- bisphosphate |
PLD1 | Phospholipase D1 |
CTGF | connective-tissue growth factor |
WISP1 | Wnt1 inducible signaling pathway protein 1 |
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Luan, Y.; Zhang, H.; Ma, K.; Liu, Y.; Lu, H.; Chen, X.; Liu, Y.; Zhang, Z. CCN3/NOV Regulates Proliferation and Neuronal Differentiation in Mouse Hippocampal Neural Stem Cells via the Activation of the Notch/PTEN/AKT Pathway. Int. J. Mol. Sci. 2023, 24, 10324. https://doi.org/10.3390/ijms241210324
Luan Y, Zhang H, Ma K, Liu Y, Lu H, Chen X, Liu Y, Zhang Z. CCN3/NOV Regulates Proliferation and Neuronal Differentiation in Mouse Hippocampal Neural Stem Cells via the Activation of the Notch/PTEN/AKT Pathway. International Journal of Molecular Sciences. 2023; 24(12):10324. https://doi.org/10.3390/ijms241210324
Chicago/Turabian StyleLuan, Yan, Hanyue Zhang, Kaige Ma, Yingfei Liu, Haixia Lu, Xinlin Chen, Yong Liu, and Zhichao Zhang. 2023. "CCN3/NOV Regulates Proliferation and Neuronal Differentiation in Mouse Hippocampal Neural Stem Cells via the Activation of the Notch/PTEN/AKT Pathway" International Journal of Molecular Sciences 24, no. 12: 10324. https://doi.org/10.3390/ijms241210324