No Evidence for Myocarditis or Other Organ Affection by Induction of an Immune Response against Critical SARS-CoV-2 Protein Epitopes in a Mouse Model Susceptible for Autoimmunity
Abstract
:1. Introduction
2. Results
2.1. Effects of the Immune Reaction against the Single B-Cell Reaction Dominant SARS-CoV-2 Spike Protein Peptides and Peptide Pools on the Myocardium
2.2. Effects of the Immune Reaction against the Single B-Cell Reaction Dominant SARS-CoV-2 Membrane Protein Peptides and Peptide Pools on the Myocardium
2.3. Effects of the Immune Reaction against the Single B-Cell Reaction Dominant SARS-CoV-2 Nucleocapsid Protein Peptides and Peptide Pools on the Myocardium
2.4. Effects of the Immune Reaction against the Single B-Cell Reaction Dominant SARS-CoV-2 Envelope Protein Peptides and Peptide Pools on the Myocardium
2.5. Effects of the Immune Reaction against the Pooled B-Cell Reaction Dominant SARS-CoV-2 Peptides on the Liver, Kidney, Lung, Intestine, and Muscle
3. Discussion
4. Materials and Methods
4.1. Peptide Establishment
4.2. Animals and Immunization with Peptides
4.3. Determination hsTnT
4.4. Histopathological Analysis
4.5. Echocardiography
4.6. Detection of Antibodies against Spike Protein Peptide Sequences via Enzyme-Linked Immunosorbent Assay (ELISA)
4.7. Detection of Albumin, Urea, Creatinine, Creatine Kinase M, and Aspartate Transaminase
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ignatz, R.M.; Zirkenbach, V.A.; Kaya, M.; Stroikova, V.; Öttl, R.; Frey, N.; Kaya, Z. No Evidence for Myocarditis or Other Organ Affection by Induction of an Immune Response against Critical SARS-CoV-2 Protein Epitopes in a Mouse Model Susceptible for Autoimmunity. Int. J. Mol. Sci. 2023, 24, 9873. https://doi.org/10.3390/ijms24129873
Ignatz RM, Zirkenbach VA, Kaya M, Stroikova V, Öttl R, Frey N, Kaya Z. No Evidence for Myocarditis or Other Organ Affection by Induction of an Immune Response against Critical SARS-CoV-2 Protein Epitopes in a Mouse Model Susceptible for Autoimmunity. International Journal of Molecular Sciences. 2023; 24(12):9873. https://doi.org/10.3390/ijms24129873
Chicago/Turabian StyleIgnatz, Rebecca Maria, Vanessa Antje Zirkenbach, Mansur Kaya, Vera Stroikova, Renate Öttl, Norbert Frey, and Ziya Kaya. 2023. "No Evidence for Myocarditis or Other Organ Affection by Induction of an Immune Response against Critical SARS-CoV-2 Protein Epitopes in a Mouse Model Susceptible for Autoimmunity" International Journal of Molecular Sciences 24, no. 12: 9873. https://doi.org/10.3390/ijms24129873