PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice
Abstract
:1. Introduction
2. Results
2.1. Two-Week VO-OHpic Treatment Improves Motor Function during EIMD in Mice
2.2. PTEN Inhibition Enhances Myofibrillar Membrane Repair during EIMD
2.3. PTEN Inhibition Upregulates MG53 to Promote Muscle Membrane Repair
2.4. PTEN Inhibition Regulates the Synthesis and Catabolism of ECM during EIMD
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. EIMD Protocol
4.3. PTEN Inhibitor Treatment
4.4. Animals Experiments
4.5. Whole-Limb Grip Strength Test
4.6. CK and LDH Level Test
4.7. Evans Blue Uptakes
4.8. Histology and Immunofluorescence Staining
4.9. Skeletal Muscle’s ECM Structure as Observed by SEM
4.10. Protein Extraction and Western Blot Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Heart | Left Kidney | Right Kidney | Liver | Spleen |
---|---|---|---|---|---|
VO | 0.167 ± 0.013 | 0.174 ± 0.01 | 0.174 ± 0.011 | 1.469 ± 0.094 | 0.081 ± 0.018 |
VC | 0.169 ± 0.01 | 0.173 ± 0.01 | 0.172 ± 0.011 | 1.475 ± 0.062 | 0.079 ± 0.018 |
Pancreas | Body weight | Soleus | Gastrocnemius | Quadriceps | |
VO | 0.155 ± 0.33 | 27.94 ± 2.68 | 0.017 ± 0.003 | 0.156 ± 0.013 | 0.239 ± 0.17 |
VC | 0.156 ± 0.21 | 27.81 ± 2.65 | 0.016 ± 0.004 | 0.153 ± 0.077 | 0.24 ± 0.023 |
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Wu, B.; Shi, L.; Wu, Y. PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice. Int. J. Mol. Sci. 2023, 24, 9954. https://doi.org/10.3390/ijms24129954
Wu B, Shi L, Wu Y. PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice. International Journal of Molecular Sciences. 2023; 24(12):9954. https://doi.org/10.3390/ijms24129954
Chicago/Turabian StyleWu, Baile, Lijun Shi, and Ying Wu. 2023. "PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice" International Journal of Molecular Sciences 24, no. 12: 9954. https://doi.org/10.3390/ijms24129954