Overexpression of a Grape MYB Transcription Factor Gene VhMYB2 Increases Salinity and Drought Tolerance in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. VhMYB2 Cloning and Phylogenetic Analysis
2.2. VhMYB2 Localization in Nucleus
2.3. Expression Level Analysis of VhMYB2 in ‘Beta’
2.4. VhMYB2 in Transgenic A. thaliana Enhanced Salinity Tolerance
2.5. VhMYB2 in Transgenic A. thaliana Activated Salinity Tolerance-Related Genes
2.6. VhMYB2 in Transgenic A. thaliana Enhanced Drought Tolerance
2.7. VhMYB2 in Transgenic A. thaliana Activated Drought Tolerance-Related Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatment Conditions
4.2. Isolation and Cloning of VhMYB2
4.3. Subcellular Localization of VhMYB2
4.4. Sequence Analysis of VhMYB2
4.5. Expression Analysis of VhMYB2
4.6. Vector Preparation of Transgenic Arabidopsis
4.7. Analysis of Related Physiological Indexes in VhMYB2-OE A. thaliana
4.8. Expression Analysis of Related-Stress Genes in VhMYB2-OE A. thaliana
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ren, C.; Li, Z.; Song, P.; Wang, Y.; Liu, W.; Zhang, L.; Li, X.; Li, W.; Han, D. Overexpression of a Grape MYB Transcription Factor Gene VhMYB2 Increases Salinity and Drought Tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 2023, 24, 10743. https://doi.org/10.3390/ijms241310743
Ren C, Li Z, Song P, Wang Y, Liu W, Zhang L, Li X, Li W, Han D. Overexpression of a Grape MYB Transcription Factor Gene VhMYB2 Increases Salinity and Drought Tolerance in Arabidopsis thaliana. International Journal of Molecular Sciences. 2023; 24(13):10743. https://doi.org/10.3390/ijms241310743
Chicago/Turabian StyleRen, Chuankun, Zhenghao Li, Penghui Song, Yu Wang, Wanda Liu, Lihua Zhang, Xingguo Li, Wenhui Li, and Deguo Han. 2023. "Overexpression of a Grape MYB Transcription Factor Gene VhMYB2 Increases Salinity and Drought Tolerance in Arabidopsis thaliana" International Journal of Molecular Sciences 24, no. 13: 10743. https://doi.org/10.3390/ijms241310743