Metagenome of Gut Microbiota Provides a Novel Insight into the Pathogenicity of Balantioides coli in Weaned Piglets
Abstract
:1. Introduction
2. Results
2.1. Enteric Pathogen Identification
2.2. Summary of Sequencing Data for the Fecal Microbiota
2.3. Diversity and Composition of Fecal Microbiota between B. coli-Positive and B. coli-Negative Piglets
2.4. Interactions among Enriched Differential Species of Gut Bacteria
2.5. Comparison of Functions of Gut Microbiota in B. coli-Colonized and B. coli-Free Piglets
2.6. Correlation between the Microbial Species and Functions
3. Discussion
4. Materials and Methods
4.1. Animals and Fecal Sample Collection
4.2. Etiological Identification of Pathogens Causing Diarrhea in Piglets
4.3. DNA Extraction and Metagenomics Sequencing
4.4. Taxonomic and Functional Annotation from Gut Metagenomes
4.5. Construction of Correlation Network
4.6. Bioinformatics Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Module | Genus | Spearman’s Correlation | p Value | Number of Genes | Coverage of Genes (%) |
---|---|---|---|---|---|
M00060: lipid A biosynthesis | Prevotella | 0.579 | 0.054 | 850 | 40.55 |
Bacteroides | 0.007 | 0.986 | 189 | 9.02 | |
Selenomonas | 0.602 | 0.042 * | 123 | 5.87 | |
Phascolarctobacterium | 0.467 | 0.148 | 98 | 4.68 | |
Parabacteroides | −0.222 | 0.571 | 48 | 2.29 | |
Mitsuokella | 0.583 | 0.052 | 45 | 2.15 | |
Alistipes | −0.593 | 0.046 * | 44 | 2.10 | |
Megasphaera | 0.600 | 0.043 * | 39 | 1.86 | |
Acidaminococcus | 0.439 | 0.185 | 35 | 1.67 | |
Clostridium | −0.781 | 0.003 ** | 33 | 1.57 | |
Ruminococcus | −0.845 | 0.001 *** | 33 | 1.57 | |
Prevotellamassilia | 0.657 | 0.022 * | 30 | 1.43 | |
Alloprevotella | 0.408 | 0.229 | 26 | 4.24 | |
Muribaculum | 0.096 | 0.883 | 24 | 1.15 | |
Roseburia | −0.282 | 0.448 | 23 | 1.10 | |
Oscillibacter | −0.719 | 0.009 ** | 22 | 1.05 | |
Eubacterium | 0.292 | 0.430 | 21 | 1.00 | |
Schwartzia | 0.067 | 0.885 | 20 | 0.95 | |
Dialister | 0.474 | 0.140 | 19 | 0.91 | |
Others | 374 | 17.84 |
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He, K.; Xiong, J.; Yang, W.; Zhao, L.; Wang, T.; Qian, W.; Hu, S.; Wang, Q.; Aleem, M.T.; Miao, W.; et al. Metagenome of Gut Microbiota Provides a Novel Insight into the Pathogenicity of Balantioides coli in Weaned Piglets. Int. J. Mol. Sci. 2023, 24, 10791. https://doi.org/10.3390/ijms241310791
He K, Xiong J, Yang W, Zhao L, Wang T, Qian W, Hu S, Wang Q, Aleem MT, Miao W, et al. Metagenome of Gut Microbiota Provides a Novel Insight into the Pathogenicity of Balantioides coli in Weaned Piglets. International Journal of Molecular Sciences. 2023; 24(13):10791. https://doi.org/10.3390/ijms241310791
Chicago/Turabian StyleHe, Kai, Jie Xiong, Wentao Yang, Lizhuo Zhao, Tianqi Wang, Weifeng Qian, Suhui Hu, Qiangqiang Wang, Muhammad Tahir Aleem, Wei Miao, and et al. 2023. "Metagenome of Gut Microbiota Provides a Novel Insight into the Pathogenicity of Balantioides coli in Weaned Piglets" International Journal of Molecular Sciences 24, no. 13: 10791. https://doi.org/10.3390/ijms241310791